Crystallization and preliminary X-ray diffraction studies of a novel killer toxin from a halotolerant yeast Pichia farinosa

A killer toxin from a halotolerant yeast, Pichia farinosa strain KK1, was crystallized at high‐ and low‐salt concentrations. Crystals from the high‐salt solution belonged to the tetragonal space group P41212 or P43212, with unit‐cell dimensions of a = b = 81.10, c = 118.46 Å. The low‐salt solution p...

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Bibliographic Details
Published in:Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 1997-01, Vol.53 (1), p.112-113
Main Authors: Kunishima, N., Kashiwagi, T., Suzuki, C., Nikkuni, S., Tsuchiya, F., Arata, Y., Morikawa, K.
Format: Article
Language:English
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Summary:A killer toxin from a halotolerant yeast, Pichia farinosa strain KK1, was crystallized at high‐ and low‐salt concentrations. Crystals from the high‐salt solution belonged to the tetragonal space group P41212 or P43212, with unit‐cell dimensions of a = b = 81.10, c = 118.46 Å. The low‐salt solution provided crystals that belonged to the same space group, with nearly same cell dimensions. Preliminary diffraction studies showed that the intensity distributions are significantly different between the two crystals. Both types of crystals contained either two or three molecules per asymmetric unit. They diffracted X‐rays beyond 2.0 Å resolution and were stable to X‐ray irradiation.
ISSN:1399-0047
0907-4449
1399-0047
DOI:10.1107/S0907444996010578