Phage diabody repertoires for selection of large numbers of bispecific antibody fragments

Methods for the generation of large numbers of different bispecific antibodies are presented. Cloning strategies are detailed to create repertoires of bispecific diabody molecules with variability on one or both of the antigen binding sites. This diabody format, when combined with the power of phage...

Full description

Saved in:
Bibliographic Details
Published in:Nature biotechnology 1996-09, Vol.14 (9), p.1149-1154
Main Authors: McGuinness, Brian T, Walter, Gerald, FitzGerald, Kevin, Schuler, Paula, Mahoney, Walt, Duncan, Alexander R, Hoogenboom, Hennie R
Format: Article
Language:English
Subjects:
Agriculture
Animals
Antibodies, Bispecific - biosynthesis
Antibodies, Bispecific - genetics
Bacteriophages - genetics
Base Sequence
Binding Sites, Antibody - genetics
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Cloning, Molecular
Digoxigenin - immunology
DNA, Recombinant - genetics
Epitopes
Fundamental and applied biological sciences. Psychology
Humans
Immunoglobulin Fragments - biosynthesis
Immunoglobulin Fragments - genetics
Life Sciences
Methods. Procedures. Technologies
Mice
Oxazolone - analogs & derivatives
Oxazolone - immunology
Polymerase Chain Reaction
Protein engineering
research-article
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Methods for the generation of large numbers of different bispecific antibodies are presented. Cloning strategies are detailed to create repertoires of bispecific diabody molecules with variability on one or both of the antigen binding sites. This diabody format, when combined with the power of phage display technology, allows the generation and analysis of thousands of different bispecific molecules. Selection for binding presumably also selects for more stable diabodies. Phage diabody libraries enable screening or selection of the best combination bispecific molecule with regards to affinity of binding, epitope recognition and pairing before manufacture of the best candidate.
ISSN:1087-0156
1546-1696
DOI:10.1038/nbt0996-1149