Effect of oocyte-sperm co-incubation on acrosome reaction in the goat

The induction of acrosome reaction of goat spermatozoa was investigated. The acrosomal status of spermatozoa was determined by a triple-staining technique. The effect of the presence of goat oocytes on the proportion of acrosome-reacted spermatozoa was also determined. Ovulated oocytes were obtained...

Full description

Saved in:
Bibliographic Details
Published in:Theriogenology 1996-07, Vol.46 (2), p.321-330
Main Authors: Martín-Lunas, E., Martino, A., Paramio, M.T., Palomo, M.J., Mogas, M.T., Bielsa, M.A., Andolz, P., Martínez, P.
Format: Article
Language:English
Subjects:
ACROSOMA
ACROSOME
acrosome reaction
CAPRIN
CAPRINOS
ESPERMATOZOO
FECONDATION
FECUNDACION
goat spermatozoa
MADURACION
MATURATION
METHODE
METODOS
OVULE
OVULO
sperm-oocyte interaction
SPERMATOZOIDE
VIABILIDAD
VIABILITE
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The induction of acrosome reaction of goat spermatozoa was investigated. The acrosomal status of spermatozoa was determined by a triple-staining technique. The effect of the presence of goat oocytes on the proportion of acrosome-reacted spermatozoa was also determined. Ovulated oocytes were obtained from superstimulated adult goats. Other sources of oocytes were adult and prepubertal goats; oocytes from both sources were maturated in vitro. There was an increase in the percentage of acrosome-reacted spermatozoa from 4% ± 0,98 to 9% ± 1,41 when oocytes from adult females were used. Similar induction rates were measured with prepubertal and adult oocytes maturated in vitro (10,4% ± 2.06 and 8.75% ± 1.06, respectively). The influence of several qualities of cumulus oophorus as well as the presence of zona pellucida was also investigated. No significant differences were obtained with any cumulus oophorus or zona pellucida oocyte complexes. Although oocyte quality is important for high fertilization rates, it does not seem to be crucial for the induction of acrosome reaction.
ISSN:0093-691X
1879-3231
DOI:10.1016/0093-691X(96)00188-4