Role of Macrophages in the Candidacidal Activity of Liposomal Amphotericin B

The role of macrophage activation in the candidacidal activity of liposome-incorporated (L) amphotericin B was investigated. Macrophages treated with L-amphotericin B killed Candida albicans more effectively than did macrophages treated with free (F) amphotericin B. However, macrophages treated with...

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Bibliographic Details
Published in:The Journal of infectious diseases 1997-01, Vol.175 (1), p.214-217
Main Authors: Mehta, Reeta T., Poddar, Sourav, Kalidas, Mamta, Gomez-Flores, Ricardo, Dulski, Kim
Format: Article
Language:English
Subjects:
Amphotericin B - administration & dosage
Amphotericin B - pharmacology
Animals
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antifungal agents
Antifungal Agents - administration & dosage
Antifungal Agents - pharmacology
Biological and medical sciences
Cancer
Candida albicans
Candida albicans - drug effects
Candida albicans - growth & development
Candida albicans - ultrastructure
Cell growth
Cells, Cultured
Concise Communications
Cultured cells
Drug Carriers
Fungal infections
Liposomes
Macrophage Activation
Macrophages
Macrophages, Peritoneal - drug effects
Macrophages, Peritoneal - immunology
Macrophages, Peritoneal - physiology
Macrophages, Peritoneal - ultrastructure
Medical sciences
Medical treatment
Mice
Microscopy, Electron, Scanning
Pharmacology. Drug treatments
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Summary:The role of macrophage activation in the candidacidal activity of liposome-incorporated (L) amphotericin B was investigated. Macrophages treated with L-amphotericin B killed Candida albicans more effectively than did macrophages treated with free (F) amphotericin B. However, macrophages treated with neither F- nor L-amphotericin B killed amphotericin B-resistant Candida tropicalis. In vivo stimulation of macrophages by intraperitoneal administration of thioglycollate, Freund's complete adjuvant, or heat-killed C. albicans followed by in vitro treatment with for L-amphotericin B, did not enhance their candidacidal activity. Intravenous administration of F- or L-amphotericin B did not augment the candidacidal activity of macrophages sensitized in vivo; however, sensitized macrophages showed enhanced killing compared with resident unstimulated cells. These studies suggest that macrophage-mediated enhancement of C. albicans killing may be due to uptake, transport, and delivery of L-amphotericin B to infected sites rather than to macrophage activation.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/175.1.214