Diagnosis of Measles with an IgM Capture EIA: The Optimal Timing of Specimen Collection after Rash Onset

The optimal timing for collection of a single serum specimen to diagnose measles by using a monoclonal antibody-capture EIA was evaluated. Results of testing paired serum samples from 166 measles cases with at least 1 IgM-positive specimen were analyzed. Among persons whose second samples were IgM-p...

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Bibliographic Details
Published in:The Journal of infectious diseases 1997-01, Vol.175 (1), p.195-199
Main Authors: Helfand, Rita F., Heath, Janet L., Anderson, Larry J., Maes, Edmond F., Guris, Dalya, Bellini, William J.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Antibodies
Antibodies, Viral - blood
Biological and medical sciences
Blood Specimen Collection
Child
Child, Preschool
Concise Communications
Diseases
Epidemiology
Exanthema
Human viral diseases
Humans
Immunoenzyme Techniques
Immunoglobulin M - blood
Infant
Infections
Infectious diseases
Measles
Measles - diagnosis
Measles Vaccine - immunology
Measles vaccines
measles virus
Measles virus - immunology
Medical sciences
Middle Aged
Preventive medicine
Specimens
Time Factors
Vaccination
Viral diseases
Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye
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Summary:The optimal timing for collection of a single serum specimen to diagnose measles by using a monoclonal antibody-capture EIA was evaluated. Results of testing paired serum samples from 166 measles cases with at least 1 IgM-positive specimen were analyzed. Among persons whose second samples were IgM-positive, the seropositivity rate for first samples was 77% when collected within 72 hand 100% when collected 4-11 days after rash onset. Among unvaccinated persons whose first samples were IgM-positive, the rate for IgM positivity of second specimens declined from 100% at 4 days to 94% at 4 weeks after rash onset, then declined further to 63% at 5 weeks. Some previously vaccinated persons became IgM-negative during the third week after rash onset. In general, a single serum specimen collected between 72 hand 4 weeks after rash onset can be used to diagnose most cases of measles with an IgM capture EIA.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/175.1.195