Intraotic Administration of Gentamicin: A New Method to Study Ototoxicity in the Crista Ampullaris of the Bullfrog

• Published in: The Laryngoscope 1997-01, Vol.107 (1), p.137-143
• Main Authors: Carranza, Angelica, Lopez, Ivan, Castellano, Philip, Hoffman, Larry, Honrubia, Vincente
• Format: Article
• Language: English
• Subjects: Administration, Topical; Animals; Anti-Bacterial Agents - administration & dosage; Biological and medical sciences; Bullfrog cristae ampullaris; Disease Models, Animal; Dose-Response Relationship, Drug; Drug toxicity and drugs side effects treatment; Gelatin Sponge, Absorbable; Gentamicin ototoxicity; Gentamicins - administration & dosage; Hair cell regeneration; Hair Cells, Vestibular - drug effects; Hair Cells, Vestibular - physiology; Medical sciences; Pharmacology. Drug treatments; Rana catesbeiana; Regeneration; Space life sciences; Toxicity: respiratory system, ent, stomatology
• ISSN: 0023-852X; 1531-4995
• DOI: 10.1097/00005537-199701000-00025

A new method of local gentamicin administration was tested in the bullfrog inner ear to achieve ototoxic‐induced hair cell destruction. Gelfoam pledgets soaked with known amounts of gentamicin were inserted into the perilymphatic cisterna of the bullfrog through a ventral surgical approach. A dose of 1.20 mg gentamicin, consistent with a perilymphatic concentration of 65 μg/ml, resulted in the desired ototoxic‐induced hair cell damage, that is, complete hair cell destruction with minimal disruption of other components of the sensory epithelium. This study demonstrates that this is a useful and simple method to investigate the process of vestibular ototoxicity and hair cell regeneration, including aspects of hair cell destruction and repair.