Human Fibroblast Adhesion to Fibrinogen

Fibrinogen and fibrin mediate the adhesion of many cell types. In this report, the adhesion sites for human dermal fibroblasts on fibrinogen are identified and characterized. Fibroblasts showed a time- and dose-dependent adhesion to fibrinogen. Using a combination of synthetic peptide mimetics, mono...

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Bibliographic Details
Published in:Biochemistry (Easton) 1997-02, Vol.36 (5), p.1123-1128
Main Authors: Farrell, David H, Al-Mondhiry, Hamid A
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Cell Adhesion - drug effects
Cells, Cultured
Fibrinogen
Fibroblasts - cytology
Fibroblasts - physiology
Humans
Infant, Newborn
Kinetics
Male
Oligopeptides - pharmacology
Recombinant Proteins
Skin - cytology
Skin Physiological Phenomena
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Summary:Fibrinogen and fibrin mediate the adhesion of many cell types. In this report, the adhesion sites for human dermal fibroblasts on fibrinogen are identified and characterized. Fibroblasts showed a time- and dose-dependent adhesion to fibrinogen. Using a combination of synthetic peptide mimetics, monoclonal antibodies, and recombinant fibrinogens, two major classes of adhesive sites were identified. One class was RGD-dependent and involved the RGD sites in the α chain of fibrinogen. αV integrins present on fibroblasts appeared to mediate this adhesion. Inhibition studies showed that the RGD-independent site was blocked by an ICAM-1 antagonist peptide. Furthermore, the inhibition was additive with RGD peptide inhibition and accounted for essentially all of the fibroblast adhesion. Together, these results suggest that fibroblast adhesion to fibrinogen is mediated by both αV integrins and ICAM-1.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi962446r