Prognostic significance of DNA ploidy and proliferative index (MIB-1 index) in gastrointestinal stromal tumors

The DNA content and proliferative index of 61 gastrointestinal stromal tumors (GIST) were measured by image analysis and correlated with the lesion's clinicopathological features and patient's survival. DNA analysis was performed on cytospin single-cell preparations obtained from the paraf...

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Bibliographic Details
Published in:Human pathology 1997-02, Vol.28 (2), p.160-165
Main Authors: Carrillo, Rosario, Candia, Antonio, Rodriguez-Peralto, Jose L, Caz, Victor
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Biological and medical sciences
Cell Division
DNA ploidy
DNA, Neoplasm - analysis
Female
Gastroenterology. Liver. Pancreas. Abdomen
Gastrointestinal Neoplasms - diagnosis
Gastrointestinal Neoplasms - mortality
gastrointestinal stromal tumor
Humans
image cytometry
Image Processing, Computer-Assisted
Ki-67 Antigen - analysis
Male
Medical sciences
Middle Aged
Paraffin Embedding
Ploidies
Prognosis
proliferation
Proportional Hazards Models
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Survival Analysis
Survival Rate
Tumors
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Summary:The DNA content and proliferative index of 61 gastrointestinal stromal tumors (GIST) were measured by image analysis and correlated with the lesion's clinicopathological features and patient's survival. DNA analysis was performed on cytospin single-cell preparations obtained from the paraffin-embedded tissue blocks. MIB-1 was the proliferation marker used on paraffin sections. DNA aneuploidy was detected in 12 tumors (18%), and high MIB-1 index (>22%) in 12 lesions (18%). DNA aneuploidy and high MIB-1 index statistically correlated with high mitotic rate (⩾5 × 10 high-power field [HPF]) ( P < .001) and with the presence of necrosis ( P < .05). The patient's survival was significantly correlated with DNA ploidy ( P < .01), MIB1 index ( P < .00001), mitotic rate ( P < .00001), presence of necrosis ( P < .0001), and size of the tumor ( P < .01). Multivariate regression analysis showed that only MIB-1 index was an independent parameter in predicting the clinical outcome for patients with GIST. The mitotic rate was the only other independent prognostic factor when MIB-1 index was not allowed to enter the model.
ISSN:0046-8177
1532-8392
DOI:10.1016/S0046-8177(97)90100-3