Human prostate-specific glandular kallikrein is expressed as an active and an inactive protein

A polymorphism in the human prostate-specific glandular kallikrein (hKLK2) gene was described by direct sequencing (by PCR) of genomic DNAs isolated from prostatic cancer tissue, benign prostatic hyperplasia tissue, and blood leukocyte specimens. Results showed two forms of human prostate-specific g...

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Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 1997-02, Vol.43 (2), p.279-284
Main Authors: Herrala, Annakaisa, Kurkela, Riitta, Porvari, Katja, Isomaki, Ritva, Henttu, Pirkko, Vihko, Pirkko
Format: Article
Language:English
Subjects:
Biological and medical sciences
DNA - chemistry
DNA - isolation & purification
Gene Expression
Genotype
Humans
Kallikreins - genetics
Kallikreins - metabolism
Leukocytes - chemistry
Male
Medical sciences
Nephrology. Urinary tract diseases
Polymerase Chain Reaction
Polymorphism, Genetic
Prostate - chemistry
Prostatic Hyperplasia - metabolism
Prostatic Neoplasms - chemistry
Recombinant Proteins
Sequence Analysis, DNA
Tissue Kallikreins
Urinary system involvement in other diseases. Miscellaneous
Urinary tract. Prostate gland
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Summary:A polymorphism in the human prostate-specific glandular kallikrein (hKLK2) gene was described by direct sequencing (by PCR) of genomic DNAs isolated from prostatic cancer tissue, benign prostatic hyperplasia tissue, and blood leukocyte specimens. Results showed two forms of human prostate-specific glandular kallikrein protein (hK2), a consequence of a change from C to T at base 792 in the hK2 coding region. Producing the two forms as recombinant proteins in insect cells demonstrated that Arg226-hK2 (CC genotype) is an active protein and Trp226-hK2 (TT genotype) is inactive. Polymorphism studies of 36 patients with prostatic diseases identified only 1 with the TT genotype. The same kind of polymorphism was not detected in the human prostate-specific antigen (hKLK3) gene. Arg226-hK2 possessed only trypsin-like enzyme activity, whereas recombinant human prostate-specific antigen (hPSA) had only chymotrypsin-like activity. Monoclonal and polyclonal antibodies raised against hPSA purified from seminal plasma detected both active and inactive hK2. Thus, because inactive as well as stable hK2 protein may be present, a lack of trypsin-like activity in hPSA standards is not enough to confirm that the materials are free of hK2 contamination.
ISSN:0009-9147
1530-8561
DOI:10.1093/clinchem/43.2.279