Capillary Electrochromatography and Capillary Electrochromatography−Mass Spectrometry for the Analysis of DNA Adduct Mixtures

Capillary electrochromatography (CEC) is an emerging technique that can be applied to the separation of neutral compound mixtures and provides a versatile alternative to micellar electrokinetic chromatography. In this paper, CEC is applied to the separation of polycyclic aromatic hydrocarbons (PAHs)...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 1997-02, Vol.69 (3), p.379-384
Main Authors: Ding, Jianmei, Vouros, Paul
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chemistry
Deoxyribonucleic acid
DNA
DNA Adducts - analysis
Electrophoresis, Capillary
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Hydrocarbons
Mass Spectrometry
Nucleic acids
Scientific imaging
Spectrophotometry, Ultraviolet
Stereoisomerism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Capillary electrochromatography (CEC) is an emerging technique that can be applied to the separation of neutral compound mixtures and provides a versatile alternative to micellar electrokinetic chromatography. In this paper, CEC is applied to the separation of polycyclic aromatic hydrocarbons (PAHs) and in vitro reaction products of PAH deoxynucleoside adducts. Some unique features related to CEC, such as convenience of stopping the flow, nanoliter flow rate, and low sample consumption, are discussed. On-column focusing in CEC can be conducted by introducing the analytes in a solution of lower solvent strength followed by elution with a stronger mobile phase, in a manner analogous to that used in normal HPLC (e.g., a 10-fold preconcentration factor can be readily achieved). Coupling of CEC to mass spectrometry for the detection of a relatively dilute DNA adduct mixture solution (10-6 M) using the on-column focusing method is also presented.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac9606968