Quantitative analysis of dopamine receptor messages in the mouse cochlea

Dopamine receptor isoforms were examined in the cochlea of the CBA J mouse by RT-PCR analysis and nucleotide sequencing, utilizing primers specific for known dopamine receptor isoforms. Cochlear cDNA sequences corresponding to dopamine D 2(long) and D 3 receptors were amplified, whereas those repres...

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Bibliographic Details
Published in:Brain research. Molecular brain research. 1997-02, Vol.44 (1), p.151-156
Main Authors: Karadaghy, Ahmad A, Lasak, John M, Chomchai, Joseph S, Khan, Khalid M, Drescher, Marian J, Drescher, Dennis G
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Cochlea
Cochlea - chemistry
Dopamine
Dopamine receptors
Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation
Fundamental and applied biological sciences. Psychology
Mice
Molecular Sequence Data
Neurotransmission
Polymerase Chain Reaction
Quantitative competitive PCR
Receptors, Dopamine - analysis
Vertebrates: nervous system and sense organs
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Summary:Dopamine receptor isoforms were examined in the cochlea of the CBA J mouse by RT-PCR analysis and nucleotide sequencing, utilizing primers specific for known dopamine receptor isoforms. Cochlear cDNA sequences corresponding to dopamine D 2(long) and D 3 receptors were amplified, whereas those representing D 1A, D 1B, D 2(short), and D 4 were not detected. Utilizing quantitative competitive PCR analysis, relative levels of dopamine receptor transcripts were found to be 0.002, 0.014, 0.016, and 1.000 for D 2(long) cochlea, D 3 cochlea, D 3 brain, and D 2(long) brain, respectively. In the context of previously published findings, the current work provides key quantitative evidence necessary to establish that dopamine is a neurotransmitter in the auditory inner ear.
ISSN:0169-328X
1872-6941
DOI:10.1016/S0169-328X(96)00261-6