The control of cell death in the early chick embryo wing bud

Developmentally programmed cell death occurs in several regions of the chick wing bud. We have studied the nature and control of this cell death in vitro in tissues from two of these regions, the posterior necrotic zone (PNZ) and the opaque patch (OP). When tissue from these regions is excised prior...

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Bibliographic Details
Published in:Poultry science 1997-01, Vol.76 (1), p.105-110
Main Authors: Maccabe, J.A. (University of Tennessee, Knoxville, TN.), Noveroske, J.K
Format: Article
Language:English
Subjects:
AILE
ALAS
Animals
Apoptosis - drug effects
Apoptosis - physiology
Chick Embryo - cytology
Chick Embryo - physiology
CHROME
CICLOHEXIMIDA
CRECIMIENTO
CROISSANCE
CROMO
CULTIVO DE ORGANOS
CULTURE D'ORGANE
CYCLOHEXIMIDE
Cycloheximide - pharmacology
Epidermal Growth Factor - pharmacology
ESTRUCTURA CELULAR
EXPERIMENTACION
EXPERIMENTACION IN VITRO
EXPERIMENTATION
EXPERIMENTATION IN VITRO
FACTEUR DE CROISSANCE IGF
FACTOR DE CRECIMIENTO IGF
Fibroblast Growth Factor 2 - pharmacology
Growth Substances - pharmacology
HORMONAS
HORMONE
In Vitro Techniques
Insulin-Like Growth Factor II - pharmacology
Mesoderm - cytology
Mesoderm - drug effects
Mesoderm - ultrastructure
Microscopy, Electron - veterinary
MORFOGENESIS
MORPHOGENESE
Necrosis
Platelet-Derived Growth Factor - pharmacology
POLLITO
POUSSIN
STRUCTURE CELLULAIRE
Transforming Growth Factor beta - pharmacology
Tumor Necrosis Factor-alpha - pharmacology
ULTRAESTRUCTURA
ULTRASTRUCTURE
Wings, Animal - cytology
Wings, Animal - embryology
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Description
Summary:Developmentally programmed cell death occurs in several regions of the chick wing bud. We have studied the nature and control of this cell death in vitro in tissues from two of these regions, the posterior necrotic zone (PNZ) and the opaque patch (OP). When tissue from these regions is excised prior to normal cell death and placed into organ culture, cell death ensues. Under these conditions, cell death in tissue from both of these regions is inhibited by fibroblast growth factor-2 (FGF-2). The only other growth factor we have found to have this function is insulin-like growth factor-II. Cell death in tissue from the OP and PNZ occurs by apoptosis, as indicated by the internucleosomal degradation of DNA and the inhibition of cell death by cycloheximide, an inhibitor of protein synthesis. If cell death is inhibited by FGF-2 and then the growth factor is washed away, a compensatory burst of cell death occurs in the PNZ tissue but not the OP tissue. This finding may indicate that in the PNZ, a death program progresses in the face of FGF-2 inhibition, resulting in more cells on the brink of death when the growth factor is removed
ISSN:0032-5791
1525-3171
DOI:10.1093/ps/76.1.105