The Effect of Space Flight on Monoclonal Antibody Synthesis in a Hybridoma Mouse Cell Line

The hybridoma cell line, 3G10G5, producing a monoclonal antibody to the major capsid protein VP1 from the avian polyomavirus budgerigar fledgling disease virus, was produced from a Balb/C mouse. This cell line was used to test the effects of microgravity on cellular processes, specifically protein s...

Full description

Saved in:
Bibliographic Details
Published in:Experimental cell research 1997-02, Vol.230 (2), p.411-414
Main Authors: Smiley, Scott A., Gillock, Eric T., Black, Maria C., Consigli, Richard A.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - biosynthesis
Antibodies, Viral - biosynthesis
Capsid - immunology
Capsid Proteins
Hybridomas - pathology
Life Sciences (General)
Mice
Mice, Inbred BALB C
Space Flight
Space life sciences
Space Simulation
Tumor Cells, Cultured
Weightlessness
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The hybridoma cell line, 3G10G5, producing a monoclonal antibody to the major capsid protein VP1 from the avian polyomavirus budgerigar fledgling disease virus, was produced from a Balb/C mouse. This cell line was used to test the effects of microgravity on cellular processes, specifically protein synthesis. A time course study utilizing incorporation of [ 35S]methionine into newly synthesized monoclonal antibody was performed on STS-77. After 5.5 days, it was observed that cell counts for the samples exposed to microgravity were lower than those of ground-based samples. However, radiolabel incorporation of the synthesized monoclonal antibody was similar in both orbiter and ground control samples. Overall, microgravity does not seem to have an effect on this cell line's ability to synthesize IgG protein.
ISSN:0014-4827
1090-2422
DOI:10.1006/excr.1996.3445