DNA Polymorphisms Among Escherichia coli Isolated from Bacteriuric Women

Restriction fragment length polymorphism (RFLP) patterns, plasmid profiles, and antimicrobial susceptibility patterns of paired sequential Escherichia coli isolates from antibiotic-treated and untreated elderly women were analyzed. Isolates from 26 of 27 subjects who were treated successfully with a...

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Bibliographic Details
Published in:The Journal of infectious diseases 1989-03, Vol.159 (3), p.526-532
Main Authors: LiPuma, John J., Stull, Terrence L., Dasen, Sue E., Pidcock, Kenneth A., Kaye, Donald, Korzeniowski, Oksana M.
Format: Article
Language:English
Subjects:
Aged
Aging
Antimicrobials
Bacteria
Bacterial diseases
Bacterial diseases of the urinary system
Bacteriuria
Bacteriuria - microbiology
Biological and medical sciences
DNA
DNA, Bacterial - genetics
DNA, Ribosomal - genetics
Escherichia coli
Escherichia coli - classification
Escherichia coli - genetics
Human bacterial diseases
Humans
Infectious diseases
Medical sciences
Microbial Sensitivity Tests
Molecular epidemiology
Older adults
Original Articles
Plasmids
Polymorphism, Restriction Fragment Length
Reinfection
RNA, Ribosomal - genetics
Urine
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Summary:Restriction fragment length polymorphism (RFLP) patterns, plasmid profiles, and antimicrobial susceptibility patterns of paired sequential Escherichia coli isolates from antibiotic-treated and untreated elderly women were analyzed. Isolates from 26 of 27 subjects who were treated successfully with antibiotics but became reinfected differed by RFLP analysis, whereas 10of 12 subjects who failed treatment and 11 of 14 untreated subjects had paired isolates with identical RFLP banding patterns. Only 40 of the 53 pairs of isolates could be analyzed by plasmid profiles; 36 of these 40 wereconcordant with RFLP analysis. Antimicrobial susceptibility patterns showed poor concordance with RFLP analysis. This study demonstrates the utility of RFLP analysis and indicates that isolation of E. coli from elderly women after sterilization of the urinary tract usually results from introduction of a new strain; elderly women who fail antibiotic therapy or receive no therapy may remain persistently infected with the same E. coli strain.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/159.3.526