Isolation of Mhc class I cDNAs from the axolotl Ambystoma mexicanum

Class I major histocompatibility complex (Mhc) cDNA clones were isolated from axolotl mRNA by polymerase chain reaction (PCR) and by screening a cDNA phage library. The nucleotide and predicted amino acid sequences show definite similarities to the Mhc class Ialpha molecules of higher vertebrates. M...

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Bibliographic Details
Published in:Immunogenetics (New York) 1997-01, Vol.45 (5), p.285-294
Main Authors: Sammut, B, Laurens, V, Tournefier, A
Format: Article
Language:English
Subjects:
Ambystoma mexicanum - genetics
Ambystoma mexicanum - immunology
Amino Acid Sequence
Animals
Base Sequence
Binding Sites
Cloning, Molecular
DNA, Complementary - genetics
Gene Expression
Genes, MHC Class I
Molecular Sequence Data
Polymorphism, Genetic
Protein Structure, Tertiary
Sequence Alignment
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Tissue Distribution
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Summary:Class I major histocompatibility complex (Mhc) cDNA clones were isolated from axolotl mRNA by polymerase chain reaction (PCR) and by screening a cDNA phage library. The nucleotide and predicted amino acid sequences show definite similarities to the Mhc class Ialpha molecules of higher vertebrates. Most of the amino acids in the peptide binding region that dock peptides at their N and C termini in mammals are conserved. Several amino acids considered to be important for the interaction of beta2-microglobulin with the Mhc alpha chain are also conserved in the axolotl sequence. The fact that axolotl class I A cDNAs are ubiquitously expressed and highly polymorphic in the alpha1 and alpha2 domains suggests the classical nature of axolotl class I A genes.
ISSN:0093-7711
1432-1211
DOI:10.1007/s002510050207