Expression of adhesion molecules and leukocyte recruitment into gastric mucosa following ischemia-reperfusion

Leukocyte infiltration is an important step in postischemic tissue damage. This study aimed to determine the expression of adhesion molecules and their relationship with leukocyte infiltration in the postischemic gastric mucosa. Gastric tissue was obtained from rats subjected to 30 min gastric ische...

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Bibliographic Details
Published in:Digestive diseases and sciences 1997-02, Vol.42 (2), p.326-332
Main Authors: ANDREWS, F. J, MALCONTENTI-WILSON, C, O'BRIEN, P. E
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Count
Gastric Mucosa - blood supply
Gastric Mucosa - metabolism
Gastric Mucosa - pathology
Gastroenterology. Liver. Pancreas. Abdomen
Immunohistochemistry
Intercellular Adhesion Molecule-1 - analysis
L-Selectin - analysis
Lymphocyte Function-Associated Antigen-1 - analysis
Male
Medical sciences
Monocytes - pathology
Neutrophils - pathology
Other diseases. Semiology
Rats
Reperfusion Injury - metabolism
Reperfusion Injury - pathology
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
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Summary:Leukocyte infiltration is an important step in postischemic tissue damage. This study aimed to determine the expression of adhesion molecules and their relationship with leukocyte infiltration in the postischemic gastric mucosa. Gastric tissue was obtained from rats subjected to 30 min gastric ischemia followed by reperfusion. Sections were stained with specific antibodies against (1) L-selectin and (2) LFA-1 on leukocytes, (3) ICAM-1 on endothelial cells, (4) PMNs, and (5) monocytes. Stained cells or blood vessels in mucosal tissue were counted using image analysis. Results showed that from 5 min of reperfusion, numbers of L-selectin-positive cells decreased, whereas LFA-1-positive cells and PMNs increased compared with controls. ICAM-1 expression did not increase until 60 min of reperfusion. Monocyte numbers were unaffected by reperfusion. We conclude that gastric ischemia-reperfusion results in a rapid influx of LFA-1-positive cells, the majority of which are PMN. L-Selectin is shed from these cells allowing them to adhere to the microvasculature via constitutively expressed ICAM-1.
ISSN:0163-2116
1573-2568
DOI:10.1023/A:1018813902094