The Use of a DNA Probe for Epidemiological Studies of Candidiasis in Immunocompromised Hosts

Reproducible typing procedures to differentiate isolates of Candida albicans are limited. C. albicans isolates were obtained from immunocompromised patients by using DNA restriction enzyme fragment analysis and hybridization with both a radiolabeled mitochondrial DNA probe and a nonradioactive (biot...

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Bibliographic Details
Published in:The Journal of infectious diseases 1989-03, Vol.159 (3), p.488-494
Main Authors: Fox, Barry C., Mobley, Harry L. T., Wade, James C.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotin
Candida albicans
Candida albicans - genetics
Candidiasis
Candidiasis - diagnosis
Candidiasis - epidemiology
Candidiasis - microbiology
Cellulose nitrate
DNA
DNA Probes
DNA, Mitochondrial - genetics
Epidemiology
Gels
General aspects
Humans
Immunocompromised hosts
man
Medical sciences
Microbiology
Mitochondrial DNA
Opportunistic Infections - epidemiology
Original Articles
Polymorphism, Restriction Fragment Length
Repetitive Sequences, Nucleic Acid
restriction fragment length polymorphism
Tumors
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Summary:Reproducible typing procedures to differentiate isolates of Candida albicans are limited. C. albicans isolates were obtained from immunocompromised patients by using DNA restriction enzyme fragment analysis and hybridization with both a radiolabeled mitochondrial DNA probe and a nonradioactive (biotinylated) DNA probe. There were 110 pathogenic and nonpathogenic C. albicans isolates from 63 immunocompromised patients. EcoRI restriction fragment analysis with the biotinylated probe revealed different “fingerprint” patterns for 60 of 63 patients. Analysis of 57 isolates from 20 patients showed no intrapatient variation regardless of the isolation site. DNA probe “fingerprint” patterns were analyzed for eight patients on serially recovered (range, 2–18 mo) C. albicans isolates. The unique patient profiles persisted over time. The application of this biotinylated C. albicans DNA probe provides a more sensitive means than simple gel restriction fragment analysis to define the epidemiology of C. albicans infection. The use of this biotinlabeled nonradioactive probe has potential application in clinical evaluations of outbreaks of nosocomial candidiasis.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/159.3.488