Amplification and Molecular Cloning of HTLV-I Sequences from DNA of Multiple Sclerosis Patients

Techniques of gene amplification, molecular cloning, and sequence analysis were used to test for the presence of sequences related to human T-lymphotropic virus type I (HTLV-I) in peripheral blood mononuclear cells of six patients with multiple sclerosis (MS) and 20 normal individuals. HTLV-I sequen...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1989-01, Vol.243 (4890), p.529-533
Main Authors: Reddy, E. Premkumar, Sandberg-Wollheim, Magnhild, Mettus, Richard V., Ray, Phillip E., DeFreitas, Elaine, Koprowski, Hilary
Format: Article
Language:English
Subjects:
Adolescent
Adult
AIDS/HIV
Analysis
Antibodies
Base Sequence
Biological and medical sciences
Blood cells
Cell lines
Child
Cloning, Molecular
DNA
DNA Restriction Enzymes
DNA, Viral - genetics
Female
Gene Amplification
Genetic aspects
Genetics
Genomes
HIV
HIV (Viruses)
Human T lymphotropic virus 1
Human T-lymphotropic virus 1 - genetics
Humans
Leukocytes
Leukocytes, Mononuclear - analysis
Leukocytes, Mononuclear - microbiology
Lymphocytes
Macrophages - analysis
Macrophages - microbiology
Male
Medical research
Medical sciences
Molecular Sequence Data
Monocytes
Multiple sclerosis
Multiple Sclerosis - microbiology
Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis
Neurology
Nucleic Acid Hybridization
Oligonucleotide Probes
Plasmids
T lymphocytes
Tests
Viruses
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Description
Summary:Techniques of gene amplification, molecular cloning, and sequence analysis were used to test for the presence of sequences related to human T-lymphotropic virus type I (HTLV-I) in peripheral blood mononuclear cells of six patients with multiple sclerosis (MS) and 20 normal individuals. HTLV-I sequences were detected in all six MS patients and in one individual from the control group by DNA blot analysis and molecular cloning of amplified DNAs. The viral sequences in MS patients were associated with adherent cell populations consisting predominantly of monocytes and macrophages. Molecular cloning and nucleotide sequence analysis indicated that these amplified viral sequences were related to the HTLV-I proviral genome.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.2536193