Specific Binding of Nuclear Proteins to the Promoter Region of a Maize Nuclear rRNA Gene Unit

The external spacer region of a nuclear rRNA gene unit of maize was analyzed for binding of nuclear proteins and revealed binding to the promoter region and to the 200-base pair repeat elements upstream of the promoter. The promoter binding site was further characterized by DNase I and exonuclease I...

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Bibliographic Details
Published in:The Journal of biological chemistry 1989-01, Vol.264 (3), p.1467-1472
Main Authors: Schmitz, M L, Maier, U G, Brown, J W S, Feix, G
Format: Article
Language:English
Subjects:
Binding, Competitive
Biological and medical sciences
Deoxyribonuclease I - metabolism
Exodeoxyribonucleases - metabolism
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
Molecular genetics
Nuclear Proteins - metabolism
Nucleic Acid Conformation
Nucleotide Mapping
Promoter Regions, Genetic
Repetitive Sequences, Nucleic Acid
RNA, Ribosomal - analysis
RNA, Ribosomal, 18S - metabolism
Transcription. Transcription factor. Splicing. Rna processing
Zea mays
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Summary:The external spacer region of a nuclear rRNA gene unit of maize was analyzed for binding of nuclear proteins and revealed binding to the promoter region and to the 200-base pair repeat elements upstream of the promoter. The promoter binding site was further characterized by DNase I and exonuclease III foot-printing as well as by gel shift and South-Western experiments, revealing the binding of several proteins. Competition experiments indicate that the protein which interacts with the repeat units also binds to the promoter region. A model for the combined action of both binding regions in the regulation of transcription is presented.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)94211-0