Adenosine kinase inhibition protects brain against transient focal ischemia in rats

• Published in: European journal of pharmacology 1997-02, Vol.320 (2), p.131-137
• Main Authors: Jiang, Ning, Kowaluk, Elizabeth A, Lee, Chi-Hung, Mazdiyasni, Hormoz, Chopp, Michael
• Format: Article
• Language: English
• Subjects: Adenosine Kinase - antagonists & inhibitors; Adenosine kinase inhibitor; Animals; Arterial Occlusive Diseases - complications; Biological and medical sciences; Body Temperature - drug effects; Body Weight - drug effects; Brain - drug effects; Brain - enzymology; Brain - physiology; Cerebral Infarction - etiology; Cerebral Infarction - prevention & control; Cerebral ischemia; Enzyme Inhibitors - therapeutic use; Ischemic Attack, Transient - prevention & control; Male; Medical sciences; Neuronal damage; Neuropharmacology; Neuroprotective agent; Neuroprotective Agents - therapeutic use; Pharmacology. Drug treatments; Rats; Rats, Wistar; Tubercidin - analogs & derivatives; Tubercidin - therapeutic use
• ISSN: 0014-2999; 1879-0712
• DOI: 10.1016/S0014-2999(96)00905-3

Endogenous adenosine released locally during cerebral ischemia is neuroprotective, and agents which decrease adenosine inactivation may potentiate its protective effects. The effects of 5′-deoxy-5-iodotubercidin (5′d-5IT), an inhibitor of the adenosine-catabolizing enzyme, adenosine kinase, were studied in male Wistar rats subjected to 2 h of transient middle cerebral artery occlusion. 5′d-5IT or the vehicle (10% DMSO in saline) was administered i.p. 30 min before, and 2 h and 6 h after the induction of middle cerebral artery occlusion. The infarct volume was determined using 2,3,5-triphenyltetrazolium chloride staining 48 h after middle cerebral artery occlusion. The infarct volume was significantly reduced in rats treated with 1.85 mg/kg×3 (57% reduction, P