Loading…

Effects of ultrasound and convection cooking to different end point temperatures on cooking characteristics, shear force and sensory properties, composition, and microscopic morphology of beef longissimus and pectoralis muscles

Longissimus and pectoralis muscles were removed from 10 steer carcasses at 4 d postmortem, aged for 14 d at 2 degrees C, then assigned to either ultrasound or convection cooking to either 62 degrees C or 70 degrees C internal end point temperature. During cooking, time-temperature profiles and energ...

Full description

Saved in:
Bibliographic Details
Published in:Journal of animal science 1997-02, Vol.75 (2), p.386-401
Main Authors: Pohlman, F.W. (Westreco, Inc., St. Joseph, MO.), Dikeman, M.E, Zayas, J.F, Unruh, J.A
Format: Article
Language:English
Subjects:
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c391t-dfc398608a11c3d45a9ddbc173c5f8b6070679882dac211531d20100cb017c7f3
cites
container_end_page 401
container_issue 2
container_start_page 386
container_title Journal of animal science
container_volume 75
creator Pohlman, F.W. (Westreco, Inc., St. Joseph, MO.)
Dikeman, M.E
Zayas, J.F
Unruh, J.A
description Longissimus and pectoralis muscles were removed from 10 steer carcasses at 4 d postmortem, aged for 14 d at 2 degrees C, then assigned to either ultrasound or convection cooking to either 62 degrees C or 70 degrees C internal end point temperature. During cooking, time-temperature profiles and energy consumption were monitored. Ultrasound cooking resulted in greater (P -.05) cooking speed, greater (P -.05) moisture retention and less (P -.05) cooking loss, greater (P -.05) efficiency of energy consumption, a more uniform cooking environment, and less (P .05) instrumental peak-force work to shear muscle samples than convection cooking. The ultrasound treatment also resulted in a reduction (P -.05) in soluble collagen content and superior (P -.05) myofibrillar tenderness, as determined by a trained sensory panel, than convection cooking. Electron micrographs indicated that ultrasound-cooked muscles had longer sarcomeres, larger diameter fibers, and more myofibrillar disruption and shattering. Longissimus muscles cooked faster (P -.05) and more (P -.05) energetically efficient, had less (P -.05) total collagen, and were superior (P -.05) in instrumental evaluated texture and sensory tenderness than pectoralis muscles. Cooking to 70 degrees C caused greater (P -.05) moisture and cooking losses, required more (P -.05) time and energy input to cook, and negatively (P -.05) affected instrumental textural and sensor tenderness characteristics. Electron micrographs indicated a shortening of sarcomeres, more deterioration of the banding structure, reduction in fiber diameter and breakdown of endomysial and perimysial connective tissue at an internal temperature of 70 degrees C vs 62 degrees. This research identifies ultrasound cooking as a new, rapid, energy-efficient method that may improve some meat textural attributes
doi_str_mv 10.2527/1997.752386x
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78853067</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>11684064</sourcerecordid><originalsourceid>FETCH-LOGICAL-c391t-dfc398608a11c3d45a9ddbc173c5f8b6070679882dac211531d20100cb017c7f3</originalsourceid><addsrcrecordid>eNpdkk2P0zAQhiMEWsrCjRMSkoWAU7v4o47jI1otH9JKHGDPluvYrYsTB08C9PfyR5i01SJxsGbkefzOjF5X1XNGr7jk6h3TWl0pyUVT_35QLZjkciVYLR5WC0o5WzUN44-rJwB7ShmXWl5UF5pKtq7ZovpzE4J3I5AcyJTGYiFPfUssHpf7n1iKucc0f4_9loyZtBEfFN-PxCMz5IjZ6LvBFztOxaPQP9ztbLFu9CXCGB0sCey8LSTk4vyxBfgecjmQoWQUGKNHxuVuyBDnvssj1EVXMrg8REe6XIZdTnl7mAfeeB9Iyv02AsRugiM-4My52BSB4JVLHp5Wj4JN4J-d42V19-Hm2_Wn1e2Xj5-v39-unNBsXLUBY1PTxjLmRLuWVrftxjElnAzNpqaK1ko3DW-t44xJwVpOGaVuQ5lyKojL6u1JF9f5MXkYTRfB-ZRs7_MERjWNFKiB4Kv_wH2eSo-zGc7QLU41R2h5gubtofhghhI7Ww6GUTMbb2bjzdl4xF-eNadN59t7-Ow01l-f6xacTaHY3kW4x_BfCK0lYm9O2C5ud79i8QY6mxKKMrO3oKThBvsh9-LEBZuN3aLD5u6rVqJeq1r8BSgC0M0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>218122092</pqid></control><display><type>article</type><title>Effects of ultrasound and convection cooking to different end point temperatures on cooking characteristics, shear force and sensory properties, composition, and microscopic morphology of beef longissimus and pectoralis muscles</title><source>Oxford Journals Online</source><creator>Pohlman, F.W. (Westreco, Inc., St. Joseph, MO.) ; Dikeman, M.E ; Zayas, J.F ; Unruh, J.A</creator><creatorcontrib>Pohlman, F.W. (Westreco, Inc., St. Joseph, MO.) ; Dikeman, M.E ; Zayas, J.F ; Unruh, J.A</creatorcontrib><description>Longissimus and pectoralis muscles were removed from 10 steer carcasses at 4 d postmortem, aged for 14 d at 2 degrees C, then assigned to either ultrasound or convection cooking to either 62 degrees C or 70 degrees C internal end point temperature. During cooking, time-temperature profiles and energy consumption were monitored. Ultrasound cooking resulted in greater (P -.05) cooking speed, greater (P -.05) moisture retention and less (P -.05) cooking loss, greater (P -.05) efficiency of energy consumption, a more uniform cooking environment, and less (P .05) instrumental peak-force work to shear muscle samples than convection cooking. The ultrasound treatment also resulted in a reduction (P -.05) in soluble collagen content and superior (P -.05) myofibrillar tenderness, as determined by a trained sensory panel, than convection cooking. Electron micrographs indicated that ultrasound-cooked muscles had longer sarcomeres, larger diameter fibers, and more myofibrillar disruption and shattering. Longissimus muscles cooked faster (P -.05) and more (P -.05) energetically efficient, had less (P -.05) total collagen, and were superior (P -.05) in instrumental evaluated texture and sensory tenderness than pectoralis muscles. Cooking to 70 degrees C caused greater (P -.05) moisture and cooking losses, required more (P -.05) time and energy input to cook, and negatively (P -.05) affected instrumental textural and sensor tenderness characteristics. Electron micrographs indicated a shortening of sarcomeres, more deterioration of the banding structure, reduction in fiber diameter and breakdown of endomysial and perimysial connective tissue at an internal temperature of 70 degrees C vs 62 degrees. This research identifies ultrasound cooking as a new, rapid, energy-efficient method that may improve some meat textural attributes</description><identifier>ISSN: 0021-8812</identifier><identifier>EISSN: 1525-3163</identifier><identifier>EISSN: 0021-8812</identifier><identifier>DOI: 10.2527/1997.752386x</identifier><identifier>PMID: 9051461</identifier><language>eng</language><publisher>Savoy, IL: Am Soc Animal Sci</publisher><subject>Animals ; APPETABILITE ; Biological and medical sciences ; CARNE DE RES ; Cattle ; COCCION ; COLAGENOS ; Collagen - analysis ; Collagen - ultrastructure ; COLLAGENE ; CONSOMMATION D'ENERGIE ; CONSUMO DE ENERGIA ; Convection ; Cooking ; Cooking - methods ; CUISSON ; DURACION ; DUREE ; Food industries ; Food Technology - methods ; Fundamental and applied biological sciences. Psychology ; Male ; Meat ; Meat and meat product industries ; MICROSCOPIA ; MICROSCOPIE ; MUSCLE ; Muscle, Skeletal - chemistry ; Muscle, Skeletal - ultrastructure ; MUSCULOS ; PALATABILIDAD ; PIECE DE VIANDE ; PIEZAS DE CARNE ; RESISTANCE MECANIQUE ; RESISTENCIA MECANICA ; SOLUBILIDAD ; SOLUBILITE ; TEMPERATURA ; TEMPERATURE ; Time Factors ; ULTRASON ; ULTRASONIDO ; Ultrasonography ; VIANDE BOVINE</subject><ispartof>Journal of animal science, 1997-02, Vol.75 (2), p.386-401</ispartof><rights>1997 INIST-CNRS</rights><rights>Copyright American Society of Animal Science Feb 1997</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-dfc398608a11c3d45a9ddbc173c5f8b6070679882dac211531d20100cb017c7f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,780,784,789,790,23930,23931,25140,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=2593995$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9051461$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pohlman, F.W. (Westreco, Inc., St. Joseph, MO.)</creatorcontrib><creatorcontrib>Dikeman, M.E</creatorcontrib><creatorcontrib>Zayas, J.F</creatorcontrib><creatorcontrib>Unruh, J.A</creatorcontrib><title>Effects of ultrasound and convection cooking to different end point temperatures on cooking characteristics, shear force and sensory properties, composition, and microscopic morphology of beef longissimus and pectoralis muscles</title><title>Journal of animal science</title><addtitle>J Anim Sci</addtitle><description>Longissimus and pectoralis muscles were removed from 10 steer carcasses at 4 d postmortem, aged for 14 d at 2 degrees C, then assigned to either ultrasound or convection cooking to either 62 degrees C or 70 degrees C internal end point temperature. During cooking, time-temperature profiles and energy consumption were monitored. Ultrasound cooking resulted in greater (P -.05) cooking speed, greater (P -.05) moisture retention and less (P -.05) cooking loss, greater (P -.05) efficiency of energy consumption, a more uniform cooking environment, and less (P .05) instrumental peak-force work to shear muscle samples than convection cooking. The ultrasound treatment also resulted in a reduction (P -.05) in soluble collagen content and superior (P -.05) myofibrillar tenderness, as determined by a trained sensory panel, than convection cooking. Electron micrographs indicated that ultrasound-cooked muscles had longer sarcomeres, larger diameter fibers, and more myofibrillar disruption and shattering. Longissimus muscles cooked faster (P -.05) and more (P -.05) energetically efficient, had less (P -.05) total collagen, and were superior (P -.05) in instrumental evaluated texture and sensory tenderness than pectoralis muscles. Cooking to 70 degrees C caused greater (P -.05) moisture and cooking losses, required more (P -.05) time and energy input to cook, and negatively (P -.05) affected instrumental textural and sensor tenderness characteristics. Electron micrographs indicated a shortening of sarcomeres, more deterioration of the banding structure, reduction in fiber diameter and breakdown of endomysial and perimysial connective tissue at an internal temperature of 70 degrees C vs 62 degrees. This research identifies ultrasound cooking as a new, rapid, energy-efficient method that may improve some meat textural attributes</description><subject>Animals</subject><subject>APPETABILITE</subject><subject>Biological and medical sciences</subject><subject>CARNE DE RES</subject><subject>Cattle</subject><subject>COCCION</subject><subject>COLAGENOS</subject><subject>Collagen - analysis</subject><subject>Collagen - ultrastructure</subject><subject>COLLAGENE</subject><subject>CONSOMMATION D'ENERGIE</subject><subject>CONSUMO DE ENERGIA</subject><subject>Convection</subject><subject>Cooking</subject><subject>Cooking - methods</subject><subject>CUISSON</subject><subject>DURACION</subject><subject>DUREE</subject><subject>Food industries</subject><subject>Food Technology - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Male</subject><subject>Meat</subject><subject>Meat and meat product industries</subject><subject>MICROSCOPIA</subject><subject>MICROSCOPIE</subject><subject>MUSCLE</subject><subject>Muscle, Skeletal - chemistry</subject><subject>Muscle, Skeletal - ultrastructure</subject><subject>MUSCULOS</subject><subject>PALATABILIDAD</subject><subject>PIECE DE VIANDE</subject><subject>PIEZAS DE CARNE</subject><subject>RESISTANCE MECANIQUE</subject><subject>RESISTENCIA MECANICA</subject><subject>SOLUBILIDAD</subject><subject>SOLUBILITE</subject><subject>TEMPERATURA</subject><subject>TEMPERATURE</subject><subject>Time Factors</subject><subject>ULTRASON</subject><subject>ULTRASONIDO</subject><subject>Ultrasonography</subject><subject>VIANDE BOVINE</subject><issn>0021-8812</issn><issn>1525-3163</issn><issn>0021-8812</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNpdkk2P0zAQhiMEWsrCjRMSkoWAU7v4o47jI1otH9JKHGDPluvYrYsTB08C9PfyR5i01SJxsGbkefzOjF5X1XNGr7jk6h3TWl0pyUVT_35QLZjkciVYLR5WC0o5WzUN44-rJwB7ShmXWl5UF5pKtq7ZovpzE4J3I5AcyJTGYiFPfUssHpf7n1iKucc0f4_9loyZtBEfFN-PxCMz5IjZ6LvBFztOxaPQP9ztbLFu9CXCGB0sCey8LSTk4vyxBfgecjmQoWQUGKNHxuVuyBDnvssj1EVXMrg8REe6XIZdTnl7mAfeeB9Iyv02AsRugiM-4My52BSB4JVLHp5Wj4JN4J-d42V19-Hm2_Wn1e2Xj5-v39-unNBsXLUBY1PTxjLmRLuWVrftxjElnAzNpqaK1ko3DW-t44xJwVpOGaVuQ5lyKojL6u1JF9f5MXkYTRfB-ZRs7_MERjWNFKiB4Kv_wH2eSo-zGc7QLU41R2h5gubtofhghhI7Ww6GUTMbb2bjzdl4xF-eNadN59t7-Ow01l-f6xacTaHY3kW4x_BfCK0lYm9O2C5ud79i8QY6mxKKMrO3oKThBvsh9-LEBZuN3aLD5u6rVqJeq1r8BSgC0M0</recordid><startdate>19970201</startdate><enddate>19970201</enddate><creator>Pohlman, F.W. (Westreco, Inc., St. Joseph, MO.)</creator><creator>Dikeman, M.E</creator><creator>Zayas, J.F</creator><creator>Unruh, J.A</creator><general>Am Soc Animal Sci</general><general>American Society of Animal Science</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>U9A</scope><scope>7X8</scope></search><sort><creationdate>19970201</creationdate><title>Effects of ultrasound and convection cooking to different end point temperatures on cooking characteristics, shear force and sensory properties, composition, and microscopic morphology of beef longissimus and pectoralis muscles</title><author>Pohlman, F.W. (Westreco, Inc., St. Joseph, MO.) ; Dikeman, M.E ; Zayas, J.F ; Unruh, J.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-dfc398608a11c3d45a9ddbc173c5f8b6070679882dac211531d20100cb017c7f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>APPETABILITE</topic><topic>Biological and medical sciences</topic><topic>CARNE DE RES</topic><topic>Cattle</topic><topic>COCCION</topic><topic>COLAGENOS</topic><topic>Collagen - analysis</topic><topic>Collagen - ultrastructure</topic><topic>COLLAGENE</topic><topic>CONSOMMATION D'ENERGIE</topic><topic>CONSUMO DE ENERGIA</topic><topic>Convection</topic><topic>Cooking</topic><topic>Cooking - methods</topic><topic>CUISSON</topic><topic>DURACION</topic><topic>DUREE</topic><topic>Food industries</topic><topic>Food Technology - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Male</topic><topic>Meat</topic><topic>Meat and meat product industries</topic><topic>MICROSCOPIA</topic><topic>MICROSCOPIE</topic><topic>MUSCLE</topic><topic>Muscle, Skeletal - chemistry</topic><topic>Muscle, Skeletal - ultrastructure</topic><topic>MUSCULOS</topic><topic>PALATABILIDAD</topic><topic>PIECE DE VIANDE</topic><topic>PIEZAS DE CARNE</topic><topic>RESISTANCE MECANIQUE</topic><topic>RESISTENCIA MECANICA</topic><topic>SOLUBILIDAD</topic><topic>SOLUBILITE</topic><topic>TEMPERATURA</topic><topic>TEMPERATURE</topic><topic>Time Factors</topic><topic>ULTRASON</topic><topic>ULTRASONIDO</topic><topic>Ultrasonography</topic><topic>VIANDE BOVINE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pohlman, F.W. (Westreco, Inc., St. Joseph, MO.)</creatorcontrib><creatorcontrib>Dikeman, M.E</creatorcontrib><creatorcontrib>Zayas, J.F</creatorcontrib><creatorcontrib>Unruh, J.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of animal science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pohlman, F.W. (Westreco, Inc., St. Joseph, MO.)</au><au>Dikeman, M.E</au><au>Zayas, J.F</au><au>Unruh, J.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of ultrasound and convection cooking to different end point temperatures on cooking characteristics, shear force and sensory properties, composition, and microscopic morphology of beef longissimus and pectoralis muscles</atitle><jtitle>Journal of animal science</jtitle><addtitle>J Anim Sci</addtitle><date>1997-02-01</date><risdate>1997</risdate><volume>75</volume><issue>2</issue><spage>386</spage><epage>401</epage><pages>386-401</pages><issn>0021-8812</issn><eissn>1525-3163</eissn><eissn>0021-8812</eissn><abstract>Longissimus and pectoralis muscles were removed from 10 steer carcasses at 4 d postmortem, aged for 14 d at 2 degrees C, then assigned to either ultrasound or convection cooking to either 62 degrees C or 70 degrees C internal end point temperature. During cooking, time-temperature profiles and energy consumption were monitored. Ultrasound cooking resulted in greater (P -.05) cooking speed, greater (P -.05) moisture retention and less (P -.05) cooking loss, greater (P -.05) efficiency of energy consumption, a more uniform cooking environment, and less (P .05) instrumental peak-force work to shear muscle samples than convection cooking. The ultrasound treatment also resulted in a reduction (P -.05) in soluble collagen content and superior (P -.05) myofibrillar tenderness, as determined by a trained sensory panel, than convection cooking. Electron micrographs indicated that ultrasound-cooked muscles had longer sarcomeres, larger diameter fibers, and more myofibrillar disruption and shattering. Longissimus muscles cooked faster (P -.05) and more (P -.05) energetically efficient, had less (P -.05) total collagen, and were superior (P -.05) in instrumental evaluated texture and sensory tenderness than pectoralis muscles. Cooking to 70 degrees C caused greater (P -.05) moisture and cooking losses, required more (P -.05) time and energy input to cook, and negatively (P -.05) affected instrumental textural and sensor tenderness characteristics. Electron micrographs indicated a shortening of sarcomeres, more deterioration of the banding structure, reduction in fiber diameter and breakdown of endomysial and perimysial connective tissue at an internal temperature of 70 degrees C vs 62 degrees. This research identifies ultrasound cooking as a new, rapid, energy-efficient method that may improve some meat textural attributes</abstract><cop>Savoy, IL</cop><pub>Am Soc Animal Sci</pub><pmid>9051461</pmid><doi>10.2527/1997.752386x</doi><tpages>16</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0021-8812
ispartof Journal of animal science, 1997-02, Vol.75 (2), p.386-401
issn 0021-8812
1525-3163
0021-8812
language eng
recordid cdi_proquest_miscellaneous_78853067
source Oxford Journals Online
subjects Animals
APPETABILITE
Biological and medical sciences
CARNE DE RES
Cattle
COCCION
COLAGENOS
Collagen - analysis
Collagen - ultrastructure
COLLAGENE
CONSOMMATION D'ENERGIE
CONSUMO DE ENERGIA
Convection
Cooking
Cooking - methods
CUISSON
DURACION
DUREE
Food industries
Food Technology - methods
Fundamental and applied biological sciences. Psychology
Male
Meat
Meat and meat product industries
MICROSCOPIA
MICROSCOPIE
MUSCLE
Muscle, Skeletal - chemistry
Muscle, Skeletal - ultrastructure
MUSCULOS
PALATABILIDAD
PIECE DE VIANDE
PIEZAS DE CARNE
RESISTANCE MECANIQUE
RESISTENCIA MECANICA
SOLUBILIDAD
SOLUBILITE
TEMPERATURA
TEMPERATURE
Time Factors
ULTRASON
ULTRASONIDO
Ultrasonography
VIANDE BOVINE
title Effects of ultrasound and convection cooking to different end point temperatures on cooking characteristics, shear force and sensory properties, composition, and microscopic morphology of beef longissimus and pectoralis muscles
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T12%3A19%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20ultrasound%20and%20convection%20cooking%20to%20different%20end%20point%20temperatures%20on%20cooking%20characteristics,%20shear%20force%20and%20sensory%20properties,%20composition,%20and%20microscopic%20morphology%20of%20beef%20longissimus%20and%20pectoralis%20muscles&rft.jtitle=Journal%20of%20animal%20science&rft.au=Pohlman,%20F.W.%20(Westreco,%20Inc.,%20St.%20Joseph,%20MO.)&rft.date=1997-02-01&rft.volume=75&rft.issue=2&rft.spage=386&rft.epage=401&rft.pages=386-401&rft.issn=0021-8812&rft.eissn=1525-3163&rft_id=info:doi/10.2527/1997.752386x&rft_dat=%3Cproquest_cross%3E11684064%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c391t-dfc398608a11c3d45a9ddbc173c5f8b6070679882dac211531d20100cb017c7f3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=218122092&rft_id=info:pmid/9051461&rfr_iscdi=true