Transcriptional Regulation of Amyloid Precursor Protein During Dibutyryl Cyclic AMP‐Induced Differentiation of NG108‐15 Cells

: Early expression of amyloid precursor protein (APP) during development of the nervous system suggests that this protein may play an important role first in axogenesis and later in synaptogenesis. To study regulation of APP mRNA expression in neuronal cells, NG108‐15 neuroblastoma × glioma cells we...

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Bibliographic Details
Published in:Journal of neurochemistry 1997-03, Vol.68 (3), p.970-978
Main Authors: Shekarabi, Masoud, Bourbonnière, Martin, Dagenais, André, Nalbantoglu, Josephine
Format: Article
Language:English
Subjects:
Amyloid beta-Protein Precursor - genetics
Amyloid beta-Protein Precursor - metabolism
Amyloid precursor protein
Animals
Biochemistry and metabolism
Biological and medical sciences
Bucladesine - pharmacology
Cell Differentiation
Central nervous system
Differentiation
Expression
Fundamental and applied biological sciences. Psychology
Half-Life
Mice
Neurons - cytology
Neurons - drug effects
RNA, Messenger - metabolism
Rodentia
Transcription
Transcription, Genetic - physiology
Tumor Cells, Cultured
Vertebrates: nervous system and sense organs
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Summary:: Early expression of amyloid precursor protein (APP) during development of the nervous system suggests that this protein may play an important role first in axogenesis and later in synaptogenesis. To study regulation of APP mRNA expression in neuronal cells, NG108‐15 neuroblastoma × glioma cells were induced to differentiate in the presence of dibutyryl cyclic AMP. Steady‐state levels of APP mRNA and APP isoforms increased gradually, concomitantly with the appearance of differentiated phenotype. Northern blot analysis showed a three‐fold increase in APP expression at day 6 of dibutyryl cyclic AMP treatment. Nuclear run‐on assays and transient transfections performed using APP promoter/reporter constructs confirmed a twofold increase in the rate of APP gene transcription. The stability of the mRNA was unchanged, with differentiated and nondifferentiated cells having the same half‐life of about 21 h. These results strongly suggest that APP mRNA induction in the differentiated NG108‐15 cells is due to an increase in the rate of transcription of the gene.
ISSN:0022-3042
1471-4159
DOI:10.1046/j.1471-4159.1997.68030970.x