Ba2+‐sensitive K+ channels in luminal‐membrane vesicles from pars convoluta of rabbit proximal tubule

This paper describes properties of 86Rb+ fluxes through a novel K+ channel in luminal‐membrane vesicles isolated from pars convoluta of rabbit proximal tubule. The uptake of 86Rb+ into potassium salt loaded vesicles was specifically inhibited by Ba2+. The isotope accumulation is driven by an electri...

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Bibliographic Details
Published in:FEBS letters 1989-01, Vol.243 (2), p.173-176
Main Authors: Jacobsen, Christian, Røigaard-Petersen, Hans, Sheikh, M.I.
Format: Article
Language:English
Subjects:
Animals
Anions - metabolism
Ba2+ sensitivity
Barium - pharmacology
Biological and medical sciences
Cations - pharmacology
Cell physiology
Electrophysiology
Fundamental and applied biological sciences. Psychology
In Vitro Techniques
K+ channel
Kidney Tubules, Proximal - metabolism
Membrane and intracellular transports
Membrane vesicle
Molecular and cellular biology
Ouabain - pharmacology
Pars convoluta
Permeability
Potassium Channels - drug effects
Potassium Channels - metabolism
Rabbits
Rubidium Radioisotopes
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Summary:This paper describes properties of 86Rb+ fluxes through a novel K+ channel in luminal‐membrane vesicles isolated from pars convoluta of rabbit proximal tubule. The uptake of 86Rb+ into potassium salt loaded vesicles was specifically inhibited by Ba2+. The isotope accumulation is driven by an electrical diffusion potential as shown in experiments using these membrane vesicles loaded with anions of different membrane permeability and was as follows: gluconate > SO2− 4 > Cl−. Furthermore, the vesicles containing the channels show a cation selectivity with the order K+ > Rb+ > Li+ > Na+ = choline+.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(89)80124-3