Limitations of alginate gels as a culture model for the study of the effects of UVA radiation on human dermal fibroblasts

Several studies were undertaken to develop three-dimensional (3-D) cell culture models that allow conditions closer to the in vivo situation. To this end, alginate gels were tested as a 3-D cell culture model that might be useful in the study of the effects of UVA on human dermal fibroblasts. Cell c...

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Bibliographic Details
Published in:Cell biology and toxicology 1997-02, Vol.13 (2), p.95-102
Main Authors: LE TALLEC, L. P, KORWIN-ZMIJOWSKA, C, ADOLPHE, M
Format: Article
Language:English
Subjects:
Alginates - pharmacology
Apoptosis
Biological and medical sciences
Biological effects of radiation
Cell culture
Cell Survival
Cells, Cultured
Culture Media
Cytotoxicity
Fibroblasts - cytology
Fibroblasts - drug effects
Fibroblasts - radiation effects
Fundamental and applied biological sciences. Psychology
Gels
Glucuronic Acid
Hexuronic Acids
Humans
Infant
Irradiation
Middle Aged
Non ionizing radiations. Hertzian waves. Biooptics
Phototoxicity
Skin - cytology
Tissues, organs and organisms biophysics
Ultraviolet radiation
Ultraviolet Rays
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Summary:Several studies were undertaken to develop three-dimensional (3-D) cell culture models that allow conditions closer to the in vivo situation. To this end, alginate gels were tested as a 3-D cell culture model that might be useful in the study of the effects of UVA on human dermal fibroblasts. Cell culture in alginate gels and the irradiation conditions were optimized. Results showed that optimized cultures in alginate gels experienced considerable cell death on UVA irradiation compared to the classical monolayer cell culture. Viability tests (cell counting and neutral red assay) were performed to show that only UVA-irradiated alginate gels were responsible for this cytotoxicity. The implication of oxygen species in the phototoxicity induced by ultraviolet light has already been described; for this reason we investigated whether oxygen species were involved in the cytotoxicity induced by alginate upon UVA irradiation. It appeared that superoxide anion is not implicated.
ISSN:0742-2091
1573-6822
DOI:10.1023/b:cbto.0000010394.58263.2c