Point Mutational Inactivation of the Retinoblastoma Antioncogene

The retinoblastoma (Rb) antioncogene encodes a nuclear phosphoprotein, p105-Rb, that forms protein complexes with the adenovirus E1A and SV40 large T oncoproteins. A novel, aberrant Rb protein detected in J82 bladder carcinoma cells was not able to form a complex with E1A and was less stable than p1...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1989-02, Vol.243 (4893), p.937-940
Main Authors: Horowitz, Jonathan M., Yandell, David W., Park, Sang-Ho, Canning, Susan, Whyte, Peter, Buchkovich, Karen, Harlow, Ed, Weinberg, Robert A., Dryja, Thaddeus P.
Format: Article
Language:English
Subjects:
Adenovirus Early Proteins
Adenoviruses
Antigens, Polyomavirus Transforming
Base Sequence
Biological and medical sciences
Biology
Carcinogenesis
Cell lines
Complementary DNA
DNA
DNA-Binding Proteins - metabolism
Exons
Eye Neoplasms - genetics
Genetic aspects
Genetics
Humans
Medical sciences
Molecular Sequence Data
Mutation
Mutation (Biology)
Oncogene Proteins, Viral - metabolism
Oncogenes
Ophthalmology
Phosphoproteins - genetics
Phosphoproteins - metabolism
Polymerase chain reaction
Proteins
Retinoblastoma
Retinoblastoma - genetics
Retinoblastoma genes
Retinoblastoma Protein
RNA
Tumors
Tumors and pseudotumors of the eye, orbit, eyelid, lacrimal apparatus
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Summary:The retinoblastoma (Rb) antioncogene encodes a nuclear phosphoprotein, p105-Rb, that forms protein complexes with the adenovirus E1A and SV40 large T oncoproteins. A novel, aberrant Rb protein detected in J82 bladder carcinoma cells was not able to form a complex with E1A and was less stable than p105-Rb. By means of a rapid method for the detection of mutations in Rb mRNA, this defective Rb protein was observed to result from a single point mutation within a splice acceptor sequence in J82 genomic DNA. This mutation eliminates a single exon and 35 amino acids from its encoded protein product.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.2521957