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Changes in dimeric inhibin A and B during normal early puberty in boys and girls
OBJECTIVE Although recently developed specific and sensitive assays of bioactive dimeric inhibin A and B have given new insights into the pituitary‐gonadal axis in adult men and during the adult female menstrual cycle, there have been no reports on circulating inhibin A and B during normal human pub...
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| Published in: | Clinical endocrinology (Oxford) 1997-01, Vol.46 (1), p.109-114 |
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| container_volume | 46 |
| creator | Crofton, P. M. Illingworth, P. J. Groome, N. P. Stirling, H. F. Swanston, I. Gow, S. Wu, F. C. W. McNeilly, A. Kelnar, C. J. H. |
| description | OBJECTIVE Although recently developed specific and sensitive assays of bioactive dimeric inhibin A and B have given new insights into the pituitary‐gonadal axis in adult men and during the adult female menstrual cycle, there have been no reports on circulating inhibin A and B during normal human puberty. The aim of this study was to assess the relationship of dimeric inhibin A and B to pubertal stage, FSH and testosterone or oestradiol in late prepuberty and in early puberty.
STUDY DESIGN AND SUBJECTS Serial samples were collected during a prospective longitudinal trial of GH treatment in short normal children. Seven boys were studied from late prepuberty to genital stage 3, and six pre‐menarche girls from late prepuberty to breast stage 4.
MEASUREMENTS Dimeric inhibin A (girls only) and inhibin B (boys and girls) were measured by highly specific and sensitive two‐site ELISAs, FSH by IRMA, testosterone and oestradiol by RIA.
RESULTS In boys, inhibin B increased progressively from pubertal stages 1 to 3 (ANOVA P |
| doi_str_mv | 10.1046/j.1365-2265.1997.d01-1744.x |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78859170</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>78859170</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5332-fa112d55e8173b29b1c0b7149f727532fb25607ab3b154f765dea8b5405214e63</originalsourceid><addsrcrecordid>eNqVkF1v0zAUhi0EGmXwE5AigbhLOLZjOxES0ihjmzSNTdrg8shOnM4lH53diObf49Cq97uyrffjHD-EfKCQUcjl53VGuRQpY1JktCxVVgNNqcrzbPeCLI7aS7IADpCClPlr8iaENQCIAtQJOSlBlELKBbldPup-ZUPi-qR2nfWuitdHZ-L7LNF9nXxL6tG7fpX0g-90m1jt2ynZjMb67TTHzDCF_86V8214S141ug323eE8JQ8_zu-Xl-n1z4ur5dl1WgnOWdpoSlkthC2o4oaVhlZgFM3LRjElOGsMExKUNtxQkTdKitrqwogcBKO5lfyUfNr3bvzwNNqwxc6Fyrat7u0wBlRFIUqqIBq_7I2VH0LwtsGNd532E1LAmSeucWaGMzOceWLkiTNP3MX0-8OY0XS2PmYPAKP-8aDrUOm28bqvXDja4ifiHrPt697217V2es4GuDy_KVgsSPcFLmzt7lig_R-UiiuBv28u8Pv9rzu4uxSo-D_QcJ8g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>78859170</pqid></control><display><type>article</type><title>Changes in dimeric inhibin A and B during normal early puberty in boys and girls</title><source>Wiley-Blackwell Read & Publish Collection</source><creator>Crofton, P. M. ; Illingworth, P. J. ; Groome, N. P. ; Stirling, H. F. ; Swanston, I. ; Gow, S. ; Wu, F. C. W. ; McNeilly, A. ; Kelnar, C. J. H.</creator><creatorcontrib>Crofton, P. M. ; Illingworth, P. J. ; Groome, N. P. ; Stirling, H. F. ; Swanston, I. ; Gow, S. ; Wu, F. C. W. ; McNeilly, A. ; Kelnar, C. J. H.</creatorcontrib><description>OBJECTIVE Although recently developed specific and sensitive assays of bioactive dimeric inhibin A and B have given new insights into the pituitary‐gonadal axis in adult men and during the adult female menstrual cycle, there have been no reports on circulating inhibin A and B during normal human puberty. The aim of this study was to assess the relationship of dimeric inhibin A and B to pubertal stage, FSH and testosterone or oestradiol in late prepuberty and in early puberty.
STUDY DESIGN AND SUBJECTS Serial samples were collected during a prospective longitudinal trial of GH treatment in short normal children. Seven boys were studied from late prepuberty to genital stage 3, and six pre‐menarche girls from late prepuberty to breast stage 4.
MEASUREMENTS Dimeric inhibin A (girls only) and inhibin B (boys and girls) were measured by highly specific and sensitive two‐site ELISAs, FSH by IRMA, testosterone and oestradiol by RIA.
RESULTS In boys, inhibin B increased progressively from pubertal stages 1 to 3 (ANOVA P<0.0001) and correlated strongly with mean testicular volume (r=0.72, P=0.0005). Prepubertal boys showed a positive correlation between inhibin B and FSH (r=0.65, P=0.056), whereas pubertal boys gave a strong negative correlation (r=0.75, P=0.012). In both prepubertal and pubertal boys positive correlations were observed between inhibin B (y) and testosterone (x) (r=0.81, P=0.008 and r=0.62, P=0.054 respectively), but the slope of the regression line between the two was much steeper before than after the onset of clinical puberty. In girls, both inhibin A and B increased through pubertal stages 1–4 (ANOVA P=0.01 and P=0.047 respectively). Both showed strong positive correlations with oestradiol (r=0.80 and 0.79, P=0.001) and with FSH (r=0.83, P=0.0004 and r=0.80, P=0.001). Inhibin A and B were also strongly correlated with each other (r=0.92, P=0.0001).
CONCLUSIONS In boys, testicular production of inhibin B increases as puberty progresses. Our results show for the first time that the initiation of puberty is accompanied by a dramatic switch from a positive to a negative relation between inhibin B and FSH as inhibin B begins to exert the expected negative feedback on FSH. The results in girls suggest that, prior to menarche, the ovarian follicles produce inhibin A and B in strict proportion, and in progressively greater amounts as puberty proceeds. Measurement of dimeric inhibin A and B may provide a sensitive new tool for determining gonadal maturity in late prepuberty and early puberty.</description><identifier>ISSN: 0300-0664</identifier><identifier>EISSN: 1365-2265</identifier><identifier>DOI: 10.1046/j.1365-2265.1997.d01-1744.x</identifier><identifier>PMID: 9059566</identifier><identifier>CODEN: CLECAP</identifier><language>eng</language><publisher>Oxford BSL: Blackwell Science Ltd</publisher><subject>Adolescent ; Biological and medical sciences ; Child ; Dimerization ; Estradiol - blood ; Female ; Follicle Stimulating Hormone - blood ; Fundamental and applied biological sciences. Psychology ; Growth Disorders - drug therapy ; Hormone metabolism and regulation ; Human Growth Hormone - therapeutic use ; Humans ; Inhibins - blood ; Male ; Peptides - blood ; Pregnancy. Parturition. Lactation ; Prospective Studies ; Prostatic Secretory Proteins ; Puberty - blood ; Regression Analysis ; Testosterone - blood ; Vertebrates: reproduction</subject><ispartof>Clinical endocrinology (Oxford), 1997-01, Vol.46 (1), p.109-114</ispartof><rights>Blackwell Science Ltd, Oxford</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5332-fa112d55e8173b29b1c0b7149f727532fb25607ab3b154f765dea8b5405214e63</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2568856$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9059566$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Crofton, P. M.</creatorcontrib><creatorcontrib>Illingworth, P. J.</creatorcontrib><creatorcontrib>Groome, N. P.</creatorcontrib><creatorcontrib>Stirling, H. F.</creatorcontrib><creatorcontrib>Swanston, I.</creatorcontrib><creatorcontrib>Gow, S.</creatorcontrib><creatorcontrib>Wu, F. C. W.</creatorcontrib><creatorcontrib>McNeilly, A.</creatorcontrib><creatorcontrib>Kelnar, C. J. H.</creatorcontrib><title>Changes in dimeric inhibin A and B during normal early puberty in boys and girls</title><title>Clinical endocrinology (Oxford)</title><addtitle>Clinical Endocrinology</addtitle><description>OBJECTIVE Although recently developed specific and sensitive assays of bioactive dimeric inhibin A and B have given new insights into the pituitary‐gonadal axis in adult men and during the adult female menstrual cycle, there have been no reports on circulating inhibin A and B during normal human puberty. The aim of this study was to assess the relationship of dimeric inhibin A and B to pubertal stage, FSH and testosterone or oestradiol in late prepuberty and in early puberty.
STUDY DESIGN AND SUBJECTS Serial samples were collected during a prospective longitudinal trial of GH treatment in short normal children. Seven boys were studied from late prepuberty to genital stage 3, and six pre‐menarche girls from late prepuberty to breast stage 4.
MEASUREMENTS Dimeric inhibin A (girls only) and inhibin B (boys and girls) were measured by highly specific and sensitive two‐site ELISAs, FSH by IRMA, testosterone and oestradiol by RIA.
RESULTS In boys, inhibin B increased progressively from pubertal stages 1 to 3 (ANOVA P<0.0001) and correlated strongly with mean testicular volume (r=0.72, P=0.0005). Prepubertal boys showed a positive correlation between inhibin B and FSH (r=0.65, P=0.056), whereas pubertal boys gave a strong negative correlation (r=0.75, P=0.012). In both prepubertal and pubertal boys positive correlations were observed between inhibin B (y) and testosterone (x) (r=0.81, P=0.008 and r=0.62, P=0.054 respectively), but the slope of the regression line between the two was much steeper before than after the onset of clinical puberty. In girls, both inhibin A and B increased through pubertal stages 1–4 (ANOVA P=0.01 and P=0.047 respectively). Both showed strong positive correlations with oestradiol (r=0.80 and 0.79, P=0.001) and with FSH (r=0.83, P=0.0004 and r=0.80, P=0.001). Inhibin A and B were also strongly correlated with each other (r=0.92, P=0.0001).
CONCLUSIONS In boys, testicular production of inhibin B increases as puberty progresses. Our results show for the first time that the initiation of puberty is accompanied by a dramatic switch from a positive to a negative relation between inhibin B and FSH as inhibin B begins to exert the expected negative feedback on FSH. The results in girls suggest that, prior to menarche, the ovarian follicles produce inhibin A and B in strict proportion, and in progressively greater amounts as puberty proceeds. Measurement of dimeric inhibin A and B may provide a sensitive new tool for determining gonadal maturity in late prepuberty and early puberty.</description><subject>Adolescent</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Dimerization</subject><subject>Estradiol - blood</subject><subject>Female</subject><subject>Follicle Stimulating Hormone - blood</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Growth Disorders - drug therapy</subject><subject>Hormone metabolism and regulation</subject><subject>Human Growth Hormone - therapeutic use</subject><subject>Humans</subject><subject>Inhibins - blood</subject><subject>Male</subject><subject>Peptides - blood</subject><subject>Pregnancy. Parturition. Lactation</subject><subject>Prospective Studies</subject><subject>Prostatic Secretory Proteins</subject><subject>Puberty - blood</subject><subject>Regression Analysis</subject><subject>Testosterone - blood</subject><subject>Vertebrates: reproduction</subject><issn>0300-0664</issn><issn>1365-2265</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqVkF1v0zAUhi0EGmXwE5AigbhLOLZjOxES0ihjmzSNTdrg8shOnM4lH53diObf49Cq97uyrffjHD-EfKCQUcjl53VGuRQpY1JktCxVVgNNqcrzbPeCLI7aS7IADpCClPlr8iaENQCIAtQJOSlBlELKBbldPup-ZUPi-qR2nfWuitdHZ-L7LNF9nXxL6tG7fpX0g-90m1jt2ynZjMb67TTHzDCF_86V8214S141ug323eE8JQ8_zu-Xl-n1z4ur5dl1WgnOWdpoSlkthC2o4oaVhlZgFM3LRjElOGsMExKUNtxQkTdKitrqwogcBKO5lfyUfNr3bvzwNNqwxc6Fyrat7u0wBlRFIUqqIBq_7I2VH0LwtsGNd532E1LAmSeucWaGMzOceWLkiTNP3MX0-8OY0XS2PmYPAKP-8aDrUOm28bqvXDja4ifiHrPt697217V2es4GuDy_KVgsSPcFLmzt7lig_R-UiiuBv28u8Pv9rzu4uxSo-D_QcJ8g</recordid><startdate>199701</startdate><enddate>199701</enddate><creator>Crofton, P. M.</creator><creator>Illingworth, P. J.</creator><creator>Groome, N. P.</creator><creator>Stirling, H. F.</creator><creator>Swanston, I.</creator><creator>Gow, S.</creator><creator>Wu, F. C. W.</creator><creator>McNeilly, A.</creator><creator>Kelnar, C. J. H.</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199701</creationdate><title>Changes in dimeric inhibin A and B during normal early puberty in boys and girls</title><author>Crofton, P. M. ; Illingworth, P. J. ; Groome, N. P. ; Stirling, H. F. ; Swanston, I. ; Gow, S. ; Wu, F. C. W. ; McNeilly, A. ; Kelnar, C. J. H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5332-fa112d55e8173b29b1c0b7149f727532fb25607ab3b154f765dea8b5405214e63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Adolescent</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Dimerization</topic><topic>Estradiol - blood</topic><topic>Female</topic><topic>Follicle Stimulating Hormone - blood</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Growth Disorders - drug therapy</topic><topic>Hormone metabolism and regulation</topic><topic>Human Growth Hormone - therapeutic use</topic><topic>Humans</topic><topic>Inhibins - blood</topic><topic>Male</topic><topic>Peptides - blood</topic><topic>Pregnancy. Parturition. Lactation</topic><topic>Prospective Studies</topic><topic>Prostatic Secretory Proteins</topic><topic>Puberty - blood</topic><topic>Regression Analysis</topic><topic>Testosterone - blood</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Crofton, P. M.</creatorcontrib><creatorcontrib>Illingworth, P. J.</creatorcontrib><creatorcontrib>Groome, N. P.</creatorcontrib><creatorcontrib>Stirling, H. F.</creatorcontrib><creatorcontrib>Swanston, I.</creatorcontrib><creatorcontrib>Gow, S.</creatorcontrib><creatorcontrib>Wu, F. C. W.</creatorcontrib><creatorcontrib>McNeilly, A.</creatorcontrib><creatorcontrib>Kelnar, C. J. H.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical endocrinology (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Crofton, P. M.</au><au>Illingworth, P. J.</au><au>Groome, N. P.</au><au>Stirling, H. F.</au><au>Swanston, I.</au><au>Gow, S.</au><au>Wu, F. C. W.</au><au>McNeilly, A.</au><au>Kelnar, C. J. H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Changes in dimeric inhibin A and B during normal early puberty in boys and girls</atitle><jtitle>Clinical endocrinology (Oxford)</jtitle><addtitle>Clinical Endocrinology</addtitle><date>1997-01</date><risdate>1997</risdate><volume>46</volume><issue>1</issue><spage>109</spage><epage>114</epage><pages>109-114</pages><issn>0300-0664</issn><eissn>1365-2265</eissn><coden>CLECAP</coden><abstract>OBJECTIVE Although recently developed specific and sensitive assays of bioactive dimeric inhibin A and B have given new insights into the pituitary‐gonadal axis in adult men and during the adult female menstrual cycle, there have been no reports on circulating inhibin A and B during normal human puberty. The aim of this study was to assess the relationship of dimeric inhibin A and B to pubertal stage, FSH and testosterone or oestradiol in late prepuberty and in early puberty.
STUDY DESIGN AND SUBJECTS Serial samples were collected during a prospective longitudinal trial of GH treatment in short normal children. Seven boys were studied from late prepuberty to genital stage 3, and six pre‐menarche girls from late prepuberty to breast stage 4.
MEASUREMENTS Dimeric inhibin A (girls only) and inhibin B (boys and girls) were measured by highly specific and sensitive two‐site ELISAs, FSH by IRMA, testosterone and oestradiol by RIA.
RESULTS In boys, inhibin B increased progressively from pubertal stages 1 to 3 (ANOVA P<0.0001) and correlated strongly with mean testicular volume (r=0.72, P=0.0005). Prepubertal boys showed a positive correlation between inhibin B and FSH (r=0.65, P=0.056), whereas pubertal boys gave a strong negative correlation (r=0.75, P=0.012). In both prepubertal and pubertal boys positive correlations were observed between inhibin B (y) and testosterone (x) (r=0.81, P=0.008 and r=0.62, P=0.054 respectively), but the slope of the regression line between the two was much steeper before than after the onset of clinical puberty. In girls, both inhibin A and B increased through pubertal stages 1–4 (ANOVA P=0.01 and P=0.047 respectively). Both showed strong positive correlations with oestradiol (r=0.80 and 0.79, P=0.001) and with FSH (r=0.83, P=0.0004 and r=0.80, P=0.001). Inhibin A and B were also strongly correlated with each other (r=0.92, P=0.0001).
CONCLUSIONS In boys, testicular production of inhibin B increases as puberty progresses. Our results show for the first time that the initiation of puberty is accompanied by a dramatic switch from a positive to a negative relation between inhibin B and FSH as inhibin B begins to exert the expected negative feedback on FSH. The results in girls suggest that, prior to menarche, the ovarian follicles produce inhibin A and B in strict proportion, and in progressively greater amounts as puberty proceeds. Measurement of dimeric inhibin A and B may provide a sensitive new tool for determining gonadal maturity in late prepuberty and early puberty.</abstract><cop>Oxford BSL</cop><pub>Blackwell Science Ltd</pub><pmid>9059566</pmid><doi>10.1046/j.1365-2265.1997.d01-1744.x</doi><tpages>6</tpages></addata></record> |
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| ispartof | Clinical endocrinology (Oxford), 1997-01, Vol.46 (1), p.109-114 |
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| subjects | Adolescent Biological and medical sciences Child Dimerization Estradiol - blood Female Follicle Stimulating Hormone - blood Fundamental and applied biological sciences. Psychology Growth Disorders - drug therapy Hormone metabolism and regulation Human Growth Hormone - therapeutic use Humans Inhibins - blood Male Peptides - blood Pregnancy. Parturition. Lactation Prospective Studies Prostatic Secretory Proteins Puberty - blood Regression Analysis Testosterone - blood Vertebrates: reproduction |
| title | Changes in dimeric inhibin A and B during normal early puberty in boys and girls |
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