Sequence-specific binding of arc repressor to DNA. Effects of operator mutations and modifications

A set of arc operators with transition and/or transversion mutations at each operator base pair has been constructed. By determining the ability of Arc to bind these variant operators, the importance of each base pair for Arc recognition has been assessed. Methylation protection experiments have als...

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Bibliographic Details
Published in:The Journal of biological chemistry 1989-02, Vol.264 (6), p.3267-3273
Main Authors: VERSHON, A. K, KELLEY, R. D, SAUER, R. T
Format: Article
Language:English
Subjects:
Bacteriophages - genetics
Base Composition
Base Sequence
Binding Sites
Biological and medical sciences
Cloning, Molecular
DNA, Viral - metabolism
Escherichia coli
Fundamental and applied biological sciences. Psychology
Methylation
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Mutation
Operator Regions, Genetic
Promoter Regions, Genetic
Repressor Proteins - genetics
Transcription Factors - genetics
Transcription. Transcription factor. Splicing. Rna processing
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Summary:A set of arc operators with transition and/or transversion mutations at each operator base pair has been constructed. By determining the ability of Arc to bind these variant operators, the importance of each base pair for Arc recognition has been assessed. Methylation protection experiments have also been used to probe points of close contact between Arc and most of the mutant operators. These data, together with phosphate interference results obtained previously for the wild type operator, provide information about the operator surface that is contacted when Arc binds.
ISSN:0021-9258
1083-351X