A novel acute lymphoid leukaemia type BCR/ABL transcript in chronic myelogenous leukaemia

Using a reverse transcription‐polymerase chain reaction (RT‐PCR), we identified a patient with typical clinical features of chronic myelogenous leukaemia (CML) in the chronic phase who showed no amplification of the CML‐type BCR/ABL transcript. RT‐PCR with primers detecting the acute lymphoid leukae...

Full description

Saved in:
Bibliographic Details
Published in:British journal of haematology 1997-03, Vol.96 (3), p.611-613
Main Authors: Okamoto, Kiyoshi, Karasawa, Masamitsu, Sakai, Hirotaka, Ogura, Hidemi, Morita, Kimio, Naruse, Takuji
Format: Article
Language:English
Subjects:
ABL
Adult
BCR
Biological and medical sciences
Chimera
chronic myelogenous leukaemia
Genes, abl - genetics
Hematologic and hematopoietic diseases
Humans
Leukemia, Myeloid, Chronic-Phase - complications
Leukemia, Myeloid, Chronic-Phase - genetics
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Male
Medical sciences
Oncogene Proteins - genetics
Philadelphia chromosome
Polymerase Chain Reaction
Precursor Cell Lymphoblastic Leukemia-Lymphoma - complications
Precursor Cell Lymphoblastic Leukemia-Lymphoma - genetics
Protein-Tyrosine Kinases
Proto-Oncogene Proteins
Proto-Oncogene Proteins c-bcr
Transcription, Genetic
translocation
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Using a reverse transcription‐polymerase chain reaction (RT‐PCR), we identified a patient with typical clinical features of chronic myelogenous leukaemia (CML) in the chronic phase who showed no amplification of the CML‐type BCR/ABL transcript. RT‐PCR with primers detecting the acute lymphoid leukaemia (ALL)‐type transcript disclosed a novel fragment co‐amplified with an ALL‐type fragment. Sequencing revealed the novel transcript to be a chimaeric mRNA produced by fusion of a segment of BCR exon 2 (e2) to ABL exon 2 (a2), with a 21 base‐pair insertion of ABL intron 1b sequence between them. This transcript has not been reported previously.
ISSN:0007-1048
1365-2141
DOI:10.1046/j.1365-2141.1997.d01-2066.x