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Outer membrane proteins of bovine Pasteurella multocida serogroup A isolates
The outer membrane proteins (OMPs) of P. multocida serotypes A3 (7 isolates), A4 (2 isolates), A3,4 and A2 (one isolate each) obtained from pneumonic cattle (10 isolates) and from one pig isolate were investigated to identify potential immunogens. SDS-PAGE of P. multocida OM isolated by SDG centrifu...
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Published in: | Veterinary microbiology 1997-02, Vol.54 (2), p.167-183 |
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Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | The outer membrane proteins (OMPs) of
P. multocida serotypes A3 (7 isolates), A4 (2 isolates), A3,4 and A2 (one isolate each) obtained from pneumonic cattle (10 isolates) and from one pig isolate were investigated to identify potential immunogens. SDS-PAGE of
P. multocida OM isolated by SDG centrifugation of spheroplasts revealed eight major OMPs. Outer membranes isolated by sarcosyl extraction or SDG had similar protein composition on Coomassie blue-stained SDS-PA gel and on immunoblots. Two major OMPs (
M
rs of 35 and 46 kDa at 100°C) demonstrated heat modifiability with apparent
M
rs of 30 and 34 kDa at 37°C, respectively. The N-terminal aa sequences of these heat modifiable proteins revealed homology with
E. coli OmpA and
Hib P1 proteins, respectively. Protease treatment of whole cells followed by western immunoblots using bovine convalescent sera identified several immunogenic, surface-exposed and conserved OMPs among the eleven
P. multocida isolates examined. The whole organism SDS-PAGE profiles of the eleven
P. multocida isolates differed such that six patterns were seen. These patterns could potentially be used as a typing system for
P. multocida bovine isolates based on the molecular weights of whole cell proteins. The above observations have potentially important implications relative to the immunity to infection. |
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ISSN: | 0378-1135 1873-2542 |
DOI: | 10.1016/S0378-1135(96)01274-6 |