Polarographic observation of substrate‐level phosphorylation and its stimulation by acetylcholine

Substrate‐level phosphorylation was observed under the conditions optimal for this process and opposite to those for oxidative phosphorylation. Polarographic registration of Ca2+ stimulated α‐ketoglutarate oxidation and self‐inhibition of uncoupled α‐ketoglutarate (KG) oxidation was used. Acetylchol...

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Bibliographic Details
Published in:FEBS letters 1989-01, Vol.243 (2), p.153-155
Main Authors: Kondrashova, M.N., Doliba, N.M.
Format: Article
Language:English
Subjects:
Acetylcholine
Acetylcholine - pharmacology
Acetylcholine - physiology
Animals
Biological and medical sciences
Calcium - pharmacology
Cell metabolism, cell oxidation
Cell physiology
Fundamental and applied biological sciences. Psychology
Guinea Pigs
In Vitro Techniques
Ketoglutaric Acids - metabolism
Male
Mitochondria
Mitochondria - metabolism
Mitochondria, Liver - metabolism
Molecular and cellular biology
Oxidation-Reduction - drug effects
Pancreas - metabolism
Phosphorylation
Polarography
Rats
Rats, Inbred Strains
Substrate-level phosphorylation
Succinates - metabolism
Succinic Acid
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Summary:Substrate‐level phosphorylation was observed under the conditions optimal for this process and opposite to those for oxidative phosphorylation. Polarographic registration of Ca2+ stimulated α‐ketoglutarate oxidation and self‐inhibition of uncoupled α‐ketoglutarate (KG) oxidation was used. Acetylcholine (ACh) administration stimulated KG oxidation and substrate‐level phosphorylation in isolated mitochondria. These effects are stronger in tissues with a higher level of endogenous acetylcholine, such as guinea pig liver vs rat liver and pancreas vs liver. The specific stimulation of KG oxidation by ACh is related to a decrease of succinate oxidation and is contrary to the specific stimulating effect of adrenaline on succinate oxidation. Therefore the existence of reciprocal hormone‐substrate‐nucleotide systems is suggested. The described set of conditions optimal for substrate‐level phosphorylation observation by polarographic registration of respiration is as convenient as the ADP test for the investigation of oxidative phosphorylation.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(89)80119-X