Isoenzyme patterns of protein kinase C and a phospholipid‐dependent but Ca2+‐inhibited enzyme fraction in the crude extracts of different tissues

We compared the protein kinase C isoenzyme patterns of crude extracts of rabbit brain, cerebellum, spleen, thymus and human and pig granulocytes. The isoenzymes were fractionated by hydroxyapatite chromatography and the protein kinase C activity was determined with a synthetic oligopeptide substrate...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 1989-01, Vol.243 (2), p.328-332
Main Authors: Faragó, Anna, Farkas, Gyöngyi, Mészáros, György, Buday, László, Antoni, Ferenc, Sepr”odi, J’anos
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Brain - enzymology
Ca2+-inhibited enzyme fraction
Calcium - pharmacology
Chromatography - methods
Diglycerides - pharmacology
Enzyme Activation - drug effects
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Granulocytes - enzymology
Humans
Isoenzyme
Isoenzymes - analysis
Isoenzymes - antagonists & inhibitors
Phosphatidylserines - pharmacology
Protein kinase C
Protein Kinase C - analysis
Protein Kinase C - antagonists & inhibitors
Rabbits
Spleen - enzymology
Swine
Synthetic oligopeptide substrate
Thymus Gland - enzymology
Tissue Extracts - analysis
Transferases
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We compared the protein kinase C isoenzyme patterns of crude extracts of rabbit brain, cerebellum, spleen, thymus and human and pig granulocytes. The isoenzymes were fractionated by hydroxyapatite chromatography and the protein kinase C activity was determined with a synthetic oligopeptide substrate. In the extracts of several tissues we also observed an enzyme fraction which was activated by phosphatidylserine+diacylglycerol but inhibited by Ca2+.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(89)80155-3