Neutrophil adhesion to fibrinogen and fibrin under flow conditions is diminished by activation and L-selectin shedding

• Published in: Blood 1997-03, Vol.89 (6), p.2131-2138
• Main Authors: KUIJPER, P. H. M, GALLARDO TORRES, H. I, VAN DER LINDEN, J. A. M, LAMMERS, J.-W. J, SIXMA, J. J, ZWAGINGA, J. J, KOENDERMAN, L
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Cell Adhesion; Cell Aggregation; Fibrin - physiology; Fibrinogen - physiology; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Hemorheology; Humans; Immunobiology; L-Selectin - metabolism; Myeloid cells: ontogeny, maturation, markers, receptors; Neutrophil Activation - physiology; Neutrophils - physiology; Polynuclears; Receptors, Leukocyte-Adhesion - physiology
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V89.6.2131

The adhesion of neutrophils (polymorphonuclear leukocytes [PMNs]) to immobilized fibrinogen/fibrin is mediated by beta2-integrins. However, the influence of physiologic flow conditions on neutrophil adhesion to these surfaces is poorly defined. In this report, the effect of flow and neutrophil activation on adhesion to immobilized fibrinogen and fibrin was examined. For the evaluation of (the distribution of) neutrophil adhesion, real-time video-assisted microscopy and custom-made software were used. Under flow conditions, adherent neutrophils appeared to support the subsequent margination of other neutrophils, thereby enhancing the adherence of these cells to fibrin. Consequently, neutrophils adhered in clusters, especially at higher shear stresses (eg, cluster index 1.4 at shear 80 mPa). Preactivation of PMNs with fMLP (10(-7) mol/L) or 4beta-phorbol, 12-myristate, 13-acetate (PMA; 100 ng/mL) resulted in approximately 50% inhibition of adhesion to fibrin and a more random distribution (cluster index