Free cyclic AMP increases in PC12 cells on depolarization

The temporal dynamics of the intracellular second messenger cyclic AMP (cAMP) were monitored in living PC12 cells by digital fluorescence ratio imaging using FlCRhR, a single‐excitation dual‐emission cAMP indicator. When the cells were depolarized by exposure to high K+, the free cAMP concentration...

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Bibliographic Details
Published in:Journal of neuroscience research 1997-03, Vol.47 (5), p.555-560
Main Authors: Agnihotri, Naveen, Kisaalita, William S., Keith, Charles H.
Format: Article
Language:English
Subjects:
Action Potentials - physiology
Animals
cyclic AMP
Cyclic AMP - physiology
depolarization
FlCRhR
Fluorescence
PC12 cells
PC12 Cells - physiology
Potassium - physiology
Rats
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Summary:The temporal dynamics of the intracellular second messenger cyclic AMP (cAMP) were monitored in living PC12 cells by digital fluorescence ratio imaging using FlCRhR, a single‐excitation dual‐emission cAMP indicator. When the cells were depolarized by exposure to high K+, the free cAMP concentration was elevated, and then slowly decreased back to resting levels when the depolarizing stimulus was removed. Furthermore, the cAMP elevation due to depolarization decreased with successive depolarizations. © 1997 Wiley‐Liss Inc.
ISSN:0360-4012
1097-4547
DOI:10.1002/(SICI)1097-4547(19970301)47:5<555::AID-JNR11>3.0.CO;2-X