The dentine disc. A review of its applicability as a model for the in vitro testing of dentine hypersensitivity

The dentine disc has been extensively used as a model for assessing potential desensitizing agents in vitro by scanning electron microscopy (SEM). Although the disc provides a readily available and reproducible test substrate, this paper addresses the problems associated with this model such as the...

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Bibliographic Details
Published in:Journal of oral rehabilitation 1997-02, Vol.24 (2), p.148-156
Main Authors: Mordan, N J, Barber, P M, Gillam, D G
Format: Article
Language:English
Subjects:
Dentin - drug effects
Dentin - ultrastructure
Dentin Sensitivity - drug therapy
Dentinal Fluid - physiology
Dentistry
Drug Combinations
Drug Evaluation - methods
Fluorides - pharmacology
Fluorides - therapeutic use
Humans
Hydrostatic Pressure
In Vitro Techniques
Materials Testing - methods
Microscopy, Electron, Scanning
Models, Structural
Nitrates - pharmacology
Nitrates - therapeutic use
Oxalates - pharmacology
Oxalates - therapeutic use
Phosphates - pharmacology
Phosphates - therapeutic use
Smear Layer
Surface Properties - drug effects
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Summary:The dentine disc has been extensively used as a model for assessing potential desensitizing agents in vitro by scanning electron microscopy (SEM). Although the disc provides a readily available and reproducible test substrate, this paper addresses the problems associated with this model such as the natural variation in the dentine tubules and the resulting differences in surface morphology. At the ultrastructural level the surface of a single etched disc exhibits variations in the size, density, orientation and extent of etching of the cut dentine tubules. In the present study a precise control procedure was designed which allowed greater correlation between test and control surfaces. Two adjacent areas of the same disc were used, one as the test surface, the other the control. Two proprietary desensitizers were examined using this methodology. This study has shown that the dentine disc is a good, reliable model for in vitro screening and testing of potential desensitizing agents, providing such controls are applied.
ISSN:0305-182X
1365-2842
DOI:10.1046/j.1365-2842.1997.d01-260.x