Hepatic regeneration in humans with various liver disease as assessed by Ki-67 staining of formalin-fixed paraffin-embedded liver tissue

: The ability to document nuclear proliferation in the liver is essential to our understanding of hepatic regeneration and hepatocellular carcinoma. While numerous tests are available to provide such information in experimental animals, few can be applied to patients with liver disease. Ki‐67 is a p...

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Bibliographic Details
Published in:Liver (Copenhagen) 1997-02, Vol.17 (1), p.13-16
Main Authors: Kaita, K. D. E., Pettigrew, N., Minuk, G. Y.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Carcinoma, Hepatocellular - metabolism
Chronic Disease
formalin-fixed paraffin-embedded
Gastroenterology. Liver. Pancreas. Abdomen
hepatic regeneration
Hepatitis - metabolism
Humans
Ki-67
Ki-67 Antigen - analysis
Liver - chemistry
Liver Cirrhosis - metabolism
Liver Diseases - metabolism
Liver Neoplasms - metabolism
Liver Regeneration - physiology
Liver. Biliary tract. Portal circulation. Exocrine pancreas
Medical sciences
Other diseases. Semiology
Retrospective Studies
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Summary:: The ability to document nuclear proliferation in the liver is essential to our understanding of hepatic regeneration and hepatocellular carcinoma. While numerous tests are available to provide such information in experimental animals, few can be applied to patients with liver disease. Ki‐67 is a proliferating nuclear antigen present in replicating cells. Recent data indicates that staining for Ki‐67 reflects nuclear proliferation in these tissues. To date, the technique has had only limited application to human liver tissues in formalin‐fixed paraffin embedded tissue. In the present study, we documented Ki‐67 staining in archival liver tissue from patients with mild chronic hepatitis, severe chronic hepatitis, inactive cirrhosis, hepatocellular carcinoma, and in normal livers. We found that Ki‐67 staining was increased in patients with mild chronic hepatitis (labelling index 29±25), severe chronic hepatitis (labelling index 41 ±40), and hepatocellular carcinoma (labelling index 71 ±61), when compared to patients with inactive cirrhosis, (labelling index 1.4±3.1), and normal livers (labelling index 2.5±3.2). In conclusion, Ki‐67 maybe a useful tool to assess hepatocyte proliferation in formalin‐fixed paraffin‐embedded human liver tissue.
ISSN:0106-9543
1600-0676
DOI:10.1111/j.1600-0676.1997.tb00772.x