Lymphocyte Immunotherapy (LI) Increases Serum Levels of Progesterone Induced Blocking Factor (PIBF)

PROBLEM: To determine if allogenic stimulation from leukocyte immunization (LI) can increase the production of an immunomodulatory protein called progesterone induced blocking factor (PIBF) by CD8+ T‐lymphocytes. METHOD: The study group consisted of 35 women, 29 who failed to conceive after repeated...

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Bibliographic Details
Published in:American journal of reproductive immunology (1989) 1997-01, Vol.37 (1), p.17-20
Main Authors: Check, Jerome H., Arwitz, Maria, Gross, Jenifer, Peymer, Mark, Szekeres-Bartho, Julia
Format: Article
Language:English
Subjects:
Abortion, Habitual - immunology
Adoptive Transfer
AIDS/HIV
Biological and medical sciences
Birth control
CD8-Positive T-Lymphocytes - metabolism
CD8-Positive T-Lymphocytes - transplantation
Embryo Transfer - adverse effects
Female
Fertilization in Vitro - adverse effects
Gynecology. Andrology. Obstetrics
Humans
Immunomodulatory protein
Isoantigens - immunology
IVF
leukocyte immunization
Male
Medical sciences
NK cells
Pregnancy
Pregnancy Proteins - biosynthesis
Pregnancy Proteins - blood
Progesterone - pharmacology
progesterone receptor
Protein Binding - drug effects
spontaneous abortion
Sterility. Assisted procreation
Suppressor Factors, Immunologic - biosynthesis
Suppressor Factors, Immunologic - blood
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Summary:PROBLEM: To determine if allogenic stimulation from leukocyte immunization (LI) can increase the production of an immunomodulatory protein called progesterone induced blocking factor (PIBF) by CD8+ T‐lymphocytes. METHOD: The study group consisted of 35 women, 29 who failed to conceive after repeated embryo transfers (ETs) and six with recurrent spontaneous abortion (RSA). The women underwent LI using the male partner's blood as the source of leukocytes. Progesterone induced blocking factor was measured pre‐ and post‐LI with an immunocytochemistry method using a PIBF‐specific polyclonal antibody. RESULTS: The mean percentage of lymphocytes expressing PIBF, as well as the percentage of cases whose PIBF level increased to 1% or more, was significantly higher post‐LI. Similarly post‐LI, there was a significantly lower percentage of zero PIBF levels. CONCLUSIONS: Leukocyte immunization causes an increase in PIBF in many cases. Possibly the improved pregnancy outcome in immunized patients with RSA or previous failure to conceive with in vitro fertilization may be partially or possibly completely explained by its stimulatory effect on PIBF.
ISSN:1046-7408
8755-8920
1600-0897
DOI:10.1111/j.1600-0897.1997.tb00188.x