Inhibitor of Hematopoietic pluripotent Stem Cell Proliferation: Purification and Determination of Its Structure

• Published in: Proceedings of the National Academy of Sciences - PNAS 1989-02, Vol.86 (3), p.779-782
• Main Authors: Lenfant, Maryse, Wdzieczak-Bakala, Joanna, Guittet, Eric, Prome, Jean-Claude, Sotty, Dominique, Frindel, Emilia
• Format: Article
• Language: English
• Subjects: 550601 - Medicine- Unsealed Radionuclides in Diagnostics; Amino acids; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; Atoms; BARYONS; Biological and medical sciences; BODY; BONE MARROW; Bone Marrow - physiology; CALVES; CATTLE; Cell cycle; Cell Cycle - drug effects; Cell cycle, cell proliferation; Cell Division - drug effects; Cell physiology; CELL PROLIFERATION; CHROMATOGRAPHY; Chromatography, High Pressure Liquid; Cytarabine - pharmacology; DNA Replication - drug effects; DOMESTIC ANIMALS; ELEMENTARY PARTICLES; FERMIONS; Fetus; Fundamental and applied biological sciences. Psychology; HADRONS; hematopoiesis; Hematopoietic Stem Cells - cytology; Hematopoietic Stem Cells - drug effects; HEMATOPOIETIC SYSTEM; Ions; LIQUID COLUMN CHROMATOGRAPHY; MAGNETIC RESONANCE; Magnetic Resonance Spectroscopy; MAMMALS; Mass Spectrometry; Mass spectroscopy; Mice; Mice, Inbred BALB C; Molecular and cellular biology; MOLECULAR STRUCTURE; Molecules; NUCLEAR MAGNETIC RESONANCE; NUCLEONS; Oligopeptides - isolation & purification; Oligopeptides - physiology; ORGANIC COMPOUNDS; ORGANS; PEPTIDES; Pluripotent stem cells; POLYPEPTIDES; PROTEINS; PROTONS; PURIFICATION; RADIOLOGY AND NUCLEAR MEDICINE; RESONANCE; RUMINANTS; SEPARATION PROCESSES; Solvents; SOMATIC CELLS; SPLEEN CELLS; STEM CELLS; TISSUES; VERTEBRATES
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.86.3.779

We report here a five-step purification procedure that led to the isolation from fetal calf bone marrow extract of a tetrapeptide, Ac-Ser-Asp-Lys-Pro (Mr 487), exerting a high inhibitory activity on the proliferation of hematopoietic pluripotent stem cells [defined here as spleen colony-forming units (CFU-S)]. The structure of this molecule was established from amino acid analysis, fast atom bombardment mass spectrometry, and 1H nuclear magnetic resonance spectral data. This structure was confirmed by comparison with the corresponding synthetic molecule, which presents identical physicochemical characteristics and biological properties. Natural and synthetic peptides administered to mice (at a dose of 100 ng per mouse) after one injection of cytosine arabinonucleoside prevent CFU-S recruitment into DNA synthesis.