Basic fibroblast growth factor-induced glycosaminoglycan production in cultured vascular endothelial cells results from enhanced protein synthesis mediated by the lipoxygenase pathway

To investigate the intracellular regulation of glycosaminoglycan (GAG) production induced by basic fibroblast growth factor (bFGF), bovine aortic endothelial cells were cultured with recombinant human bFGF in the presence of [ 3H]glucosamine or [ 35S]sulfate. It was shown that bFGF-induced incorpora...

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Bibliographic Details
Published in:Life sciences (1973) 1997, Vol.60 (12), p.873-881
Main Authors: Kaji, Toshiyuki, Miyamoto, Akiko, Yamamoto, Chika, Fujiwara, Yasuyuki, Miyajima, Sawako, Koizumi, Fumitomo
Format: Article
Language:English
Subjects:
Animals
Aorta - cytology
basic fibroblast growth factor
Cattle
Cells, Cultured
endothelial cell
Endothelium, Vascular - metabolism
Fibroblast Growth Factor 2 - pharmacology
Glycosaminoglycans - biosynthesis
Humans
Indomethacin - pharmacology
lipoxygenase
Lipoxygenase - metabolism
Lipoxygenase Inhibitors - pharmacology
Masoprocol - pharmacology
Sulfates - metabolism
vascular system
xylosyl transferase
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Summary:To investigate the intracellular regulation of glycosaminoglycan (GAG) production induced by basic fibroblast growth factor (bFGF), bovine aortic endothelial cells were cultured with recombinant human bFGF in the presence of [ 3H]glucosamine or [ 35S]sulfate. It was shown that bFGF-induced incorporation of the radioactive precursors into GAGs was diminished by lipoxygenase inhibitors, nordihydroguaiaretic acid (NDGA) and esculetin, but not by a cyclooxygenase inhibitor indomethacin. A protein synthesis inhibitor cycloheximide also diminished the enhancement of the [ 3H]glucosamine incorporation by bFGF. On the other hand, the incorporation of [ 14C]leucine into the acid-insoluble fraction was strongly inhibited by NDGA but not by indomethacin in the presence or absence of bFGF. It was also shown that bFGF significantly increased the incorporation of [ 14C]xylose into GAGs. The present data suggested that bFGF may increase the number of GAG chains as a result of enhanced protein synthesis including xylosyl transferase through the lipoxygenase pathway of arachidonic acid metabolism in vascular endothelial cell layer.
ISSN:0024-3205
1879-0631
DOI:10.1016/S0024-3205(97)00019-2