Reconstitution of periplasmic transport in inside-out membrane vesicles. Energization by ATP

• Published in: The Journal of biological chemistry 1989-03, Vol.264 (7), p.3998-4002
• Main Authors: Ames, G F, Nikaido, K, Groarke, J, Petithory, J
• Format: Article
• Language: English
• Subjects: Adenosine Triphosphate - physiology; Biological Transport, Active; Cell Membrane - metabolism; Escherichia coli - physiology; Histidine - metabolism; In Vitro Techniques; Kinetics; liposomes; Membrane Transport Proteins - physiology; Salmonella typhimurium; Salmonella typhimurium - physiology
• ISSN: 0021-9258; 1083-351X

The periplasmic histidine permease of Salmonella typhimurium has been reconstituted in inside-out vesicles (IOV) of Escherichia coli by disrupting the cells with a French press in the presence of a high concentration of the periplasmic histidine-binding protein, HisJ. Efflux from IOV, which is equivalent to uptake in whole cells, is induced by ATP. The reconstituted system depends on the presence of the membrane-bound permease proteins, HisQ, HisM, and HisP, and does not function if reconstitution is performed in the presence of a mutant HisJ protein, HisJ5625, that can bind histidine normally but can't interact properly with the membrane complex. Efflux is not induced by the nonhydrolyzable ATP analog, adenyl-5'-yl imidodiphosphate, supporting the contention that ATP hydrolysis is necessary. 8-Azido ATP inactivates IOV, indicating that the ATP effect occurs through the HisP protein, which has previously been shown to be modified by 8-azido ATP (Hobson, A., Weatherwax, R., and Ames, G.F.-L. (1984) Proc. Natl. Acad. Sci. U. S. A. 81, 733-7337). The estimated Km of the vesicles for ATP is about 200 microM. Vanadate, an inhibitor of phosphohydrolase enzymes, inhibits ATP-induced efflux. We conclude that ATP is likely to be the proximal energy source for periplasmic permeases.