Activation of phosphatidylcholine-selective phospholipase C by I1-imidazoline receptors in PC12 cells and rostral ventrolateral medulla

The I1-imidazoline receptor is expressed in the rostral ventrolateral medulla (RVLM) where it mediates vasodepression, and in PC12 pheochromocytoma cells where it elicits generation of diacylglycerol independent of phosphatidylinositol turnover or activation of phospholipase D. We hypothesized that...

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Bibliographic Details
Published in:Brain research 1997-02, Vol.749 (2), p.335-339
Main Authors: Separovic, D, Kester, M, Haxhiu, M A, Ernsberger, P
Format: Article
Language:English
Subjects:
Adrenal Gland Neoplasms
Animals
Antihypertensive Agents - pharmacology
Bridged-Ring Compounds - administration & dosage
Bridged-Ring Compounds - pharmacology
Diglycerides - metabolism
Enzyme Activation
Imidazoles - pharmacology
Imidazoline Receptors
Medulla Oblongata - enzymology
Medulla Oblongata - physiology
Microinjections
Models, Biological
Nerve Growth Factors - pharmacology
PC12 Cells
Pheochromocytoma
Rats
Rats, Inbred SHR
Receptors, Drug - agonists
Receptors, Drug - antagonists & inhibitors
Receptors, Drug - physiology
Signal Transduction
Thiones - administration & dosage
Thiones - pharmacology
Type C Phospholipases - antagonists & inhibitors
Type C Phospholipases - metabolism
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Summary:The I1-imidazoline receptor is expressed in the rostral ventrolateral medulla (RVLM) where it mediates vasodepression, and in PC12 pheochromocytoma cells where it elicits generation of diacylglycerol independent of phosphatidylinositol turnover or activation of phospholipase D. We hypothesized that the I1-imidazoline receptor couples to a phosphatidylcholine-selective phospholipase C (PC-PLC). The I1-agonist moxonidine elicited diacyglyceride accumulation and release of [3H]phosphocholine from PC12 cells prelabeled with [3H]choline. The PC-PLC inhibitor D609 abolished both responses. Microinjection of D609 into the RVLM of hypertensive rats blocked the vasodepressor response to intravenous moxonidine. These data implicate PC-PLC in cellular and organismic responses to I1-receptor stimulation.
ISSN:0006-8993
DOI:10.1016/S0006-8993(96)01372-8