IgE Memory B Cells Identified by the Polymerase Chain Reaction

The existence of memory B cells for IgE was investigated using spleen cells harvested from mice primed and boosted with dinitrophenylated keyhole limpet hemocyanin. Using the polymerase chain reaction, it was found that mRNA for IgE was already demonstrable in spleen cells taken 1 day after boost. S...

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Bibliographic Details
Published in:Cellular immunology 1997-02, Vol.176 (1), p.34-40
Main Authors: Takahama, Hideto, Furusawa, Shuichi, Ovary, Zoltan
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic
Animals
B-Lymphocytes - immunology
Cell Separation
Cells, Cultured
Female
Hemocyanins - immunology
Immunoglobulin E - analysis
Immunoglobulin E - biosynthesis
Immunologic Memory
Mice
Mice, Inbred BALB C
Polymerase Chain Reaction
Spleen - cytology
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Summary:The existence of memory B cells for IgE was investigated using spleen cells harvested from mice primed and boosted with dinitrophenylated keyhole limpet hemocyanin. Using the polymerase chain reaction, it was found that mRNA for IgE was already demonstrable in spleen cells taken 1 day after boost. Spleen cells taken from mice 1 day after boost and culturedin vitrowithout the addition of antigen also produced anti-dinitrophenylated (DNP) IgE antibody. In contrast, mRNA for IgE in spleen cells from mice primed 4 weeks previously, but not boosted, was not demonstrable. Anti-DNP IgE was not produced in cultures of spleen cells from mice primed but not boosted. As mRNA for IgE was demonstrable already 1 day after boost we concluded that mice produce memory B cells not only for IgM, but also for IgE specific for the immunizing antigen.
ISSN:0008-8749
1090-2163
DOI:10.1006/cimm.1996.1065