Morphological analysis of degeneration and regeneration of syncytiotrophoblast in first trimester placental villi during organ culture

We have recently shown using dansyl-L-lysine exclusion studies that the release of human chorionic gonadotrophin (HCG) in conjunction with L-lactate dehydrogenase (LDH) from first trimester villi during organ culture is symptomatic of syncytiotrophoblast degeneration. The purpose of this study was t...

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Bibliographic Details
Published in:Human reproduction (Oxford) 1997-02, Vol.12 (2), p.379-382
Main Authors: Palmer, M E, Watson, A L, Burton, G J
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chorionic Villi - ultrastructure
Embryology: invertebrates and vertebrates. Teratology
Female
Fetal membranes
Fundamental and applied biological sciences. Psychology
General aspects. Development. Fetal membranes
Humans
Microscopy, Electron
Organ Culture Techniques
Pregnancy
Pregnancy Trimester, First
Regeneration
Trophoblasts - physiology
Trophoblasts - ultrastructure
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Summary:We have recently shown using dansyl-L-lysine exclusion studies that the release of human chorionic gonadotrophin (HCG) in conjunction with L-lactate dehydrogenase (LDH) from first trimester villi during organ culture is symptomatic of syncytiotrophoblast degeneration. The purpose of this study was to examine chorionic villi at the ultrastructural level in order to determine events occurring during organ culture. The tissue was sampled after 0, 24, 48 and 120 h in culture and processed for electron microscopy. In addition to confirming the previously recorded syncytial degeneration, the electron micrographs showed clearly the generation of a new syncytiotrophoblast layer. The new layer, derived from differentiating cytotrophoblast cells, was largely formed by 48 h and was maintained for at least 120 h in culture. This study demonstrates a model which provides an opportunity to study the differentiation of cytotrophoblast cells whilst they retain their anatomical relationships within the villous structure.
ISSN:0268-1161
1460-2350
DOI:10.1093/humrep/12.2.379