Novel gene delivery to liver cells using engineered virosomes

We have demonstrated for the first time that the reconstituted Sendai viral envelopes containing only the fusion protein (F-virosomes) are efficient vehicles for the delivery of foreign genes specifically into human hepatoblastoma cells (HepG2) in culture. The membrane fusion-mediated entry of CAT (...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 1997-03, Vol.404 (2), p.164-168
Main Authors: Ramani, Komal, Bora, Roop S, Kumar, Mukesh, Tyagi, Sandeep K, Sarkar, Debi P
Format: Article
Language:English
Subjects:
alpha-Fetoproteins
ampicillin
Animals
Asialoglycoproteins
CAT
chloramphenicol acetyl transferase
Chloramphenicol O-Acetyltransferase - biosynthesis
CHO Cells
CMV
Cricetinae
cytomegalovirus
dhfr
dihydrofolate reductase
DMEM
DPBS
Dulbecco's modified Eagle's medium
Dulbecco's phosphate-buffered saline
ELISA
enzyme-linked immunosorbent assay
FCS
fetal calf serum
Fetuins
fusion protein
Gene delivery
Gene therapy
Genes, Reporter
Genetic Engineering - methods
Genetic Therapy - methods
Genetic Vectors
hemagglutinin-neuraminidase
Hepatoblastoma
Humans
Kinetics
Liver Neoplasms
Membrane fusion
PBS
phenylmethylsulphonylfluoride
phosphate-buffered saline
PMSF
RBC
reconstituted Sendai viral envelope
red blood cell(s)
Respirovirus
RSVE
SDS-PAGE
Sendai virus
simian virus 40
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
SV40
TBS
Transfection - methods
Tris-buffered saline
Tris-EDTA
Tumor Cells, Cultured
Viral Fusion Proteins
Virosome
WGA
wheat germ agglutinin
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have demonstrated for the first time that the reconstituted Sendai viral envelopes containing only the fusion protein (F-virosomes) are efficient vehicles for the delivery of foreign genes specifically into human hepatoblastoma cells (HepG2) in culture. The membrane fusion-mediated entry of CAT (chloramphenicol acetyl transferase) gene into the cells was confirmed and the amount delivered to various subcellular fractions was quantitated. The dose dependence and kinetics of expression of biologically active CAT protein in HepG2 cells was measured. The CAT expression level in F-virosome-mediated delivery was significantly higher than that of Lipofectin or liganded proteo-liposome-mediated gene transfer. This kind of targeted delivery by means of membrane fusion induced by viral envelope glycoprotein may have wide applications to various gene transfer strategies both in vitro and in vivo. © 1997 Federation of European Biochemical Societies.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(97)00120-8