Membrane responses of B-16 melanoma cells to single exposure to ultraviolet light

Electron spin resonance spectroscopy using the spin probe (5-, 12- and 16-deoxylstearic acid) was employed to analyze the changes in membrane fluidity in B-16 melanoma cells following UV-B exposure. The UV exposure resulted in the immediate accumulation of lipid peroxide, being accompanied by a chan...

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Bibliographic Details
Published in:Archives of Dermatological Research 1989, Vol.280 (8), p.481-486
Main Authors: Ogura, R, Sugiyama, M, Sakanashi, T, Suematsu, T, Hidaka, T, Morikawa, F, Kon, Y
Format: Article
Language:English
Subjects:
Arachidonic Acid
Arachidonic Acids - metabolism
Arachidonic Acids - radiation effects
Electron Spin Resonance Spectroscopy
Humans
Lipid Peroxidation - radiation effects
Melanocytes - metabolism
Melanocytes - radiation effects
Melanoma, Experimental - metabolism
Membrane Fluidity - radiation effects
Membrane Lipids - radiation effects
Tumor Cells, Cultured
Ultraviolet Rays
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Summary:Electron spin resonance spectroscopy using the spin probe (5-, 12- and 16-deoxylstearic acid) was employed to analyze the changes in membrane fluidity in B-16 melanoma cells following UV-B exposure. The UV exposure resulted in the immediate accumulation of lipid peroxide, being accompanied by a change in membrane fluidity. The 12-DSA is the most sensitive to the changes in membrane organization caused by UV light. Na+,K+-ATPase activity was regulated by a change in membrane fluidity. Following UV exposure, the release of the prelabeled arachidonic acid from the cells was observed immediately. Ca2+-dependent calmodulin-dependent phospholipase A2-like activity was involved in the UV-stimulated arachidonic acid release from phospholipid.
ISSN:0340-3696
1432-069X
DOI:10.1007/BF00427661