Characterization of polymorphisms in the toxin A and B genes of Clostridium difficile

Abstract We have used six independent polymerase chain reactions (A1–A3 and B1–B3) for amplification of the entire sequence of the two toxin genes tcdA and tcdB of several Clostridium difficile strains. With this approach we have detected (1) restriction site polymorphisms which are distributed all...

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Bibliographic Details
Published in:FEMS microbiology letters 1997-03, Vol.148 (2), p.197-202
Main Authors: Rupnik, Maja, Braun, Veit, Soehn, Florian, Janc, Miha, Hofstetter, Monika, Laufenberg-Feldmann, Rita, von Eichel-Streiber, Christoph
Format: Article
Language:English
Subjects:
Bacterial Proteins
Bacterial Toxins - genetics
Clostridium difficile
Clostridium difficile - genetics
Enterotoxins - genetics
Genes
Genes, Bacterial
Genetic polymorphism
Microbiology
Polymerase Chain Reaction
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
Toxin A
Toxin B
Toxins
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Summary:Abstract We have used six independent polymerase chain reactions (A1–A3 and B1–B3) for amplification of the entire sequence of the two toxin genes tcdA and tcdB of several Clostridium difficile strains. With this approach we have detected (1) restriction site polymorphisms which are distributed all over the genes, and (2) deletions that could be found only in tcdA. Characteristic differences between strains were mainly focused to the 5′ third of tcdB (B1 fragment) and/or the 3′ third of tcdA (A3 fragment). The possible use of our approach for typing of C. difficile toxin genes is discussed.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1997.tb10288.x