Pharmacokinetics of a Chinese traditional medicine, Danshensu (3,4-dihydroxyphenyllactic acid), in rabbits using high-performance liquid chromatography

An injectable solution of Danshen was prepared and its in vivo disposition was examined in rabbits. The presence of Danshensu, one of the active components of Danshen, in the obtained solution was confirmed by a simple high-performance liquid chromatographic (HPLC) method. The pharmacokinetics of Da...

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Bibliographic Details
Published in:Biological & pharmaceutical bulletin 1997, Vol.20 (3), p.285-287
Main Authors: ZHAO, F. Q, ZHENG, N. X, SATO, H, ADACHI, I, HORIKOSHI, I
Format: Article
Language:English
Subjects:
Animals
Area Under Curve
Biological and medical sciences
Chromatography, High Pressure Liquid
Drugs, Chinese Herbal - administration & dosage
Drugs, Chinese Herbal - pharmacokinetics
General pharmacology
Half-Life
Injections, Intravenous
Lactates - administration & dosage
Lactates - pharmacokinetics
Male
Medical sciences
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Rabbits
Spectrophotometry, Ultraviolet
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Summary:An injectable solution of Danshen was prepared and its in vivo disposition was examined in rabbits. The presence of Danshensu, one of the active components of Danshen, in the obtained solution was confirmed by a simple high-performance liquid chromatographic (HPLC) method. The pharmacokinetics of Danshensu in rabbits was evaluated by the HPLC method for plasma Danshensu. The calibration curve for Danshensu was linear (r = 0.998) over the concentration range of 0.25-40.0 micrograms/ml. The intra-assay coefficients of variation (CVs) were 3.8, 3.1, and 3.1% at 1, 10, and 50 micrograms/ml, respectively, and the inter-assay CVs were 5.3, 5.3, and 2.9% at 1, 10, and 50 micrograms/ml, respectively. The analytical recovery of Danshensu in plasma averaged 95.2%. From the plasma concentration profile of Danshensu after its intravenous administration, the t1/2, mean residence time (MRT), Vdss, and Cltot were determined as 32 min, 48 min, 149 ml/kg, and 3.13 ml/min/kg, respectively.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.20.285