Assignment of the murine protein kinase gene DLK to chromosome 15 in the vicinity of the bt/Koa locus by genetic linkage analysis

We have cloned protein kinase genes from murine primordial germ cell-derived EG cells by a PCR-based strategy using degenerate primers corresponding to the conserved sequences in the catalytic domain of protein kinases. One of these clones, designated Gek (germ cell kinase) 2, was used as a probe fo...

Full description

Saved in:
Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 1997-03, Vol.40 (2), p.375-376
Main Authors: WATANABE, T, YANAGISAWA, M, MATSUBARA, N, OBINATA, M, MATSUI, Y
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Chromosome Mapping
Classical genetics, quantitative genetics, hybrids
Fundamental and applied biological sciences. Psychology
Genetic Linkage
Genetics of eukaryotes. Biological and molecular evolution
MAP Kinase Kinase Kinases
Mice
Polymorphism, Restriction Fragment Length
Protein-Serine-Threonine Kinases - genetics
Vertebrata
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have cloned protein kinase genes from murine primordial germ cell-derived EG cells by a PCR-based strategy using degenerate primers corresponding to the conserved sequences in the catalytic domain of protein kinases. One of these clones, designated Gek (germ cell kinase) 2, was used as a probe for screening of a mouse brain cDNA library, and obtained clones contained an entire coding sequence. Comparison of the sequence of Gek2 with those in databases revealed that it was identical to a previously reported protein kinase gene, DLK.
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.1996.4565