Specific Expression of CPP32 in Sensory Neurons of Mouse Embryos and Activation of CPP32 in the Apoptosis Induced by a Withdrawal of NGF

We isolated mouse CPP32/apopain cDNA, a mammalian homologue most closely related toCed-3inC. elegans,and examined the involvement of CPP32 in the apoptosis of nervous system during development. CPP32 is specifically expressed in the trigeminal (V) ganglia, facio-acoustic (VII-VIII) ganglion complex,...

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Published in:Biochemical and biophysical research communications 1997-02, Vol.231 (3), p.770-774
Main Authors: Mukasa, Takeshi, Urase, Koko, Momoi, Mariko Y., Kimura, Ichiroh, Momoi, Takashi
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Apoptosis - drug effects
Caspase 3
Caspases
Cricetinae
Cysteine Endopeptidases - genetics
Enzyme Activation
Ganglia, Spinal - enzymology
Gene Expression Regulation
Humans
In Situ Hybridization
Mice
Molecular Sequence Data
Nerve Growth Factors - pharmacology
Neurons, Afferent - enzymology
RNA, Messenger - genetics
Sequence Alignment
Sequence Homology, Amino Acid
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Summary:We isolated mouse CPP32/apopain cDNA, a mammalian homologue most closely related toCed-3inC. elegans,and examined the involvement of CPP32 in the apoptosis of nervous system during development. CPP32 is specifically expressed in the trigeminal (V) ganglia, facio-acoustic (VII-VIII) ganglion complex, and dorsal root ganglia (DRGs) of mouse 10.5-day embryos. CPP32-like proteases are activated during apoptosis of DRG neurons induced by deprivation of NGF and serum. Ac-DEVD-CHO, an inhibitor for CPP32-like proteases, prevents apoptosis of DRG neurons, but Ac-YVAD-CHO, an inhibitor for ICE-like proteases, does not. These results suggest that CPP32 or CPP32-like proteases play a role as central mediator in the apoptosis of DRG neurons induced by lack of neurotrophin signals.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1996.6002