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Inositol-phosphoglycan inhibits calcium oscillations in hepatocytes by reducing calcium entry
Inositol-phosphoglycan (IPG) is a putative mediator of insulin action that has been shown to affect numerous biochemical processes. IPG, prepared from liver membranes, promptly inhibited phenylephrine- or vasopressin-induced [Ca 2+] i oscillations when perfused over Fura-2-dextran injected rat hepat...
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| Published in: | Cell calcium (Edinburgh) 1997-02, Vol.21 (2), p.125-133 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c391t-e9d6579b8067381c1b1a0923ad4462eb70ca1688c9a052d33802a718260573633 |
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| cites | cdi_FETCH-LOGICAL-c391t-e9d6579b8067381c1b1a0923ad4462eb70ca1688c9a052d33802a718260573633 |
| container_end_page | 133 |
| container_issue | 2 |
| container_start_page | 125 |
| container_title | Cell calcium (Edinburgh) |
| container_volume | 21 |
| creator | Sanchez-Bueno, Antonio Greenwood, Mark R. Varela-Nieto, Isabel Marrero, Isabel Gil, Beatriz Mato, Jose M. Cobbold, Peter H. |
| description | Inositol-phosphoglycan (IPG) is a putative mediator of insulin action that has been shown to affect numerous biochemical processes. IPG, prepared from liver membranes, promptly inhibited phenylephrine- or vasopressin-induced [Ca
2+]
i oscillations when perfused over Fura-2-dextran injected rat hepatocytes. An antibody to IPG suppressed the inhibitory effect of insulin on the [Ca
2+]
i oscillations. Measurement of the rate of quench of cytoplasmic Fura-2 by extracellular Mn
2+ showed that Ca
2+ entry occurred continuously in the unstimulated cell and was not affected by phenylephrine or vasopressin. IPG, specifically, almost completely abolished the Mn
2+ quench rate. Elevated extracellular [Ca
2+] reversed the inhibitory effect of IPG on [Ca
2+]
i oscillations. We conclude that IPG inhibits the hepatocyte Ca
2+ oscillator by reducing the continuous Ca
2+ influx that is required to sustain oscillations in [Ca
2+]
i. |
| doi_str_mv | 10.1016/S0143-4160(97)90036-1 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78895316</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0143416097900361</els_id><sourcerecordid>15913400</sourcerecordid><originalsourceid>FETCH-LOGICAL-c391t-e9d6579b8067381c1b1a0923ad4462eb70ca1688c9a052d33802a718260573633</originalsourceid><addsrcrecordid>eNqF0E9LwzAYBvAgis7pRxB6Ej1U8zZt0pxEhv9g4EE9SkjTVxfpmpmkQr-9mRu7egg5PM-bN_wIOQN6BRT49QuFkuUlcHohxaWklPEc9sgEKlbkICXsk8muckSOQ_iilEom4JAcSmBFIasJeX_qXbDRdflq4UI6n91odJ_ZfmEbG0NmdGfssMxcMLbrdLSuDynNFrjS0ZkxYsiaMfPYDsb2n7s-9tGPJ-TgQ3cBT7f3lLzd373OHvP588PT7HaeGyYh5ihbXgnZ1JQLVoOBBjSVBdNtWfICG0GNBl7XRmpaFS1jNS20gLrgtBKMMzYl55t3V959DxiiWtpgMP23RzcEJepaVgz4v0WokkyZLKek2hSNdyF4_FArb5fajwqoWvurP3-1xlVSqD9_BWnubLtgaJbY7qa24Cm_2eSYOH4sepVcsTfYWo8mqtbZfzb8AsL8lOU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15913400</pqid></control><display><type>article</type><title>Inositol-phosphoglycan inhibits calcium oscillations in hepatocytes by reducing calcium entry</title><source>ScienceDirect Freedom Collection 2022-2024</source><creator>Sanchez-Bueno, Antonio ; Greenwood, Mark R. ; Varela-Nieto, Isabel ; Marrero, Isabel ; Gil, Beatriz ; Mato, Jose M. ; Cobbold, Peter H.</creator><creatorcontrib>Sanchez-Bueno, Antonio ; Greenwood, Mark R. ; Varela-Nieto, Isabel ; Marrero, Isabel ; Gil, Beatriz ; Mato, Jose M. ; Cobbold, Peter H.</creatorcontrib><description>Inositol-phosphoglycan (IPG) is a putative mediator of insulin action that has been shown to affect numerous biochemical processes. IPG, prepared from liver membranes, promptly inhibited phenylephrine- or vasopressin-induced [Ca
2+]
i oscillations when perfused over Fura-2-dextran injected rat hepatocytes. An antibody to IPG suppressed the inhibitory effect of insulin on the [Ca
2+]
i oscillations. Measurement of the rate of quench of cytoplasmic Fura-2 by extracellular Mn
2+ showed that Ca
2+ entry occurred continuously in the unstimulated cell and was not affected by phenylephrine or vasopressin. IPG, specifically, almost completely abolished the Mn
2+ quench rate. Elevated extracellular [Ca
2+] reversed the inhibitory effect of IPG on [Ca
2+]
i oscillations. We conclude that IPG inhibits the hepatocyte Ca
2+ oscillator by reducing the continuous Ca
2+ influx that is required to sustain oscillations in [Ca
2+]
i.</description><identifier>ISSN: 0143-4160</identifier><identifier>EISSN: 1532-1991</identifier><identifier>DOI: 10.1016/S0143-4160(97)90036-1</identifier><identifier>PMID: 9132295</identifier><language>eng</language><publisher>Netherlands: Elsevier India Pvt Ltd</publisher><subject>Animals ; Calcium - metabolism ; Cells, Cultured ; Chlorides - pharmacology ; Fluorescent Dyes ; Fura-2 ; Inositol Phosphates - pharmacology ; Insulin - pharmacology ; Insulin Antagonists - pharmacology ; Liver - cytology ; Liver - metabolism ; Male ; Manganese - metabolism ; Manganese Compounds - pharmacology ; Microinjections ; Nitrous Acid - pharmacology ; Phenylephrine - pharmacology ; Polysaccharides - pharmacology ; Rats ; Rats, Wistar ; Vasopressins - pharmacology</subject><ispartof>Cell calcium (Edinburgh), 1997-02, Vol.21 (2), p.125-133</ispartof><rights>1997</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-e9d6579b8067381c1b1a0923ad4462eb70ca1688c9a052d33802a718260573633</citedby><cites>FETCH-LOGICAL-c391t-e9d6579b8067381c1b1a0923ad4462eb70ca1688c9a052d33802a718260573633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9132295$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sanchez-Bueno, Antonio</creatorcontrib><creatorcontrib>Greenwood, Mark R.</creatorcontrib><creatorcontrib>Varela-Nieto, Isabel</creatorcontrib><creatorcontrib>Marrero, Isabel</creatorcontrib><creatorcontrib>Gil, Beatriz</creatorcontrib><creatorcontrib>Mato, Jose M.</creatorcontrib><creatorcontrib>Cobbold, Peter H.</creatorcontrib><title>Inositol-phosphoglycan inhibits calcium oscillations in hepatocytes by reducing calcium entry</title><title>Cell calcium (Edinburgh)</title><addtitle>Cell Calcium</addtitle><description>Inositol-phosphoglycan (IPG) is a putative mediator of insulin action that has been shown to affect numerous biochemical processes. IPG, prepared from liver membranes, promptly inhibited phenylephrine- or vasopressin-induced [Ca
2+]
i oscillations when perfused over Fura-2-dextran injected rat hepatocytes. An antibody to IPG suppressed the inhibitory effect of insulin on the [Ca
2+]
i oscillations. Measurement of the rate of quench of cytoplasmic Fura-2 by extracellular Mn
2+ showed that Ca
2+ entry occurred continuously in the unstimulated cell and was not affected by phenylephrine or vasopressin. IPG, specifically, almost completely abolished the Mn
2+ quench rate. Elevated extracellular [Ca
2+] reversed the inhibitory effect of IPG on [Ca
2+]
i oscillations. We conclude that IPG inhibits the hepatocyte Ca
2+ oscillator by reducing the continuous Ca
2+ influx that is required to sustain oscillations in [Ca
2+]
i.</description><subject>Animals</subject><subject>Calcium - metabolism</subject><subject>Cells, Cultured</subject><subject>Chlorides - pharmacology</subject><subject>Fluorescent Dyes</subject><subject>Fura-2</subject><subject>Inositol Phosphates - pharmacology</subject><subject>Insulin - pharmacology</subject><subject>Insulin Antagonists - pharmacology</subject><subject>Liver - cytology</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Manganese - metabolism</subject><subject>Manganese Compounds - pharmacology</subject><subject>Microinjections</subject><subject>Nitrous Acid - pharmacology</subject><subject>Phenylephrine - pharmacology</subject><subject>Polysaccharides - pharmacology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Vasopressins - pharmacology</subject><issn>0143-4160</issn><issn>1532-1991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqF0E9LwzAYBvAgis7pRxB6Ej1U8zZt0pxEhv9g4EE9SkjTVxfpmpmkQr-9mRu7egg5PM-bN_wIOQN6BRT49QuFkuUlcHohxaWklPEc9sgEKlbkICXsk8muckSOQ_iilEom4JAcSmBFIasJeX_qXbDRdflq4UI6n91odJ_ZfmEbG0NmdGfssMxcMLbrdLSuDynNFrjS0ZkxYsiaMfPYDsb2n7s-9tGPJ-TgQ3cBT7f3lLzd373OHvP588PT7HaeGyYh5ihbXgnZ1JQLVoOBBjSVBdNtWfICG0GNBl7XRmpaFS1jNS20gLrgtBKMMzYl55t3V959DxiiWtpgMP23RzcEJepaVgz4v0WokkyZLKek2hSNdyF4_FArb5fajwqoWvurP3-1xlVSqD9_BWnubLtgaJbY7qa24Cm_2eSYOH4sepVcsTfYWo8mqtbZfzb8AsL8lOU</recordid><startdate>19970201</startdate><enddate>19970201</enddate><creator>Sanchez-Bueno, Antonio</creator><creator>Greenwood, Mark R.</creator><creator>Varela-Nieto, Isabel</creator><creator>Marrero, Isabel</creator><creator>Gil, Beatriz</creator><creator>Mato, Jose M.</creator><creator>Cobbold, Peter H.</creator><general>Elsevier India Pvt Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>19970201</creationdate><title>Inositol-phosphoglycan inhibits calcium oscillations in hepatocytes by reducing calcium entry</title><author>Sanchez-Bueno, Antonio ; Greenwood, Mark R. ; Varela-Nieto, Isabel ; Marrero, Isabel ; Gil, Beatriz ; Mato, Jose M. ; Cobbold, Peter H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-e9d6579b8067381c1b1a0923ad4462eb70ca1688c9a052d33802a718260573633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Calcium - metabolism</topic><topic>Cells, Cultured</topic><topic>Chlorides - pharmacology</topic><topic>Fluorescent Dyes</topic><topic>Fura-2</topic><topic>Inositol Phosphates - pharmacology</topic><topic>Insulin - pharmacology</topic><topic>Insulin Antagonists - pharmacology</topic><topic>Liver - cytology</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Manganese - metabolism</topic><topic>Manganese Compounds - pharmacology</topic><topic>Microinjections</topic><topic>Nitrous Acid - pharmacology</topic><topic>Phenylephrine - pharmacology</topic><topic>Polysaccharides - pharmacology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Vasopressins - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sanchez-Bueno, Antonio</creatorcontrib><creatorcontrib>Greenwood, Mark R.</creatorcontrib><creatorcontrib>Varela-Nieto, Isabel</creatorcontrib><creatorcontrib>Marrero, Isabel</creatorcontrib><creatorcontrib>Gil, Beatriz</creatorcontrib><creatorcontrib>Mato, Jose M.</creatorcontrib><creatorcontrib>Cobbold, Peter H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell calcium (Edinburgh)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sanchez-Bueno, Antonio</au><au>Greenwood, Mark R.</au><au>Varela-Nieto, Isabel</au><au>Marrero, Isabel</au><au>Gil, Beatriz</au><au>Mato, Jose M.</au><au>Cobbold, Peter H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inositol-phosphoglycan inhibits calcium oscillations in hepatocytes by reducing calcium entry</atitle><jtitle>Cell calcium (Edinburgh)</jtitle><addtitle>Cell Calcium</addtitle><date>1997-02-01</date><risdate>1997</risdate><volume>21</volume><issue>2</issue><spage>125</spage><epage>133</epage><pages>125-133</pages><issn>0143-4160</issn><eissn>1532-1991</eissn><abstract>Inositol-phosphoglycan (IPG) is a putative mediator of insulin action that has been shown to affect numerous biochemical processes. IPG, prepared from liver membranes, promptly inhibited phenylephrine- or vasopressin-induced [Ca
2+]
i oscillations when perfused over Fura-2-dextran injected rat hepatocytes. An antibody to IPG suppressed the inhibitory effect of insulin on the [Ca
2+]
i oscillations. Measurement of the rate of quench of cytoplasmic Fura-2 by extracellular Mn
2+ showed that Ca
2+ entry occurred continuously in the unstimulated cell and was not affected by phenylephrine or vasopressin. IPG, specifically, almost completely abolished the Mn
2+ quench rate. Elevated extracellular [Ca
2+] reversed the inhibitory effect of IPG on [Ca
2+]
i oscillations. We conclude that IPG inhibits the hepatocyte Ca
2+ oscillator by reducing the continuous Ca
2+ influx that is required to sustain oscillations in [Ca
2+]
i.</abstract><cop>Netherlands</cop><pub>Elsevier India Pvt Ltd</pub><pmid>9132295</pmid><doi>10.1016/S0143-4160(97)90036-1</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0143-4160 |
| ispartof | Cell calcium (Edinburgh), 1997-02, Vol.21 (2), p.125-133 |
| issn | 0143-4160 1532-1991 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78895316 |
| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | Animals Calcium - metabolism Cells, Cultured Chlorides - pharmacology Fluorescent Dyes Fura-2 Inositol Phosphates - pharmacology Insulin - pharmacology Insulin Antagonists - pharmacology Liver - cytology Liver - metabolism Male Manganese - metabolism Manganese Compounds - pharmacology Microinjections Nitrous Acid - pharmacology Phenylephrine - pharmacology Polysaccharides - pharmacology Rats Rats, Wistar Vasopressins - pharmacology |
| title | Inositol-phosphoglycan inhibits calcium oscillations in hepatocytes by reducing calcium entry |
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