Structure of the O-specific polysaccharide of Proteus penneri strain 25 containing N-(L-alanyl) and multiple O-acetyl groups in a tetrasaccharide repeating unit

Based on sugar and methylation analyses, O-deacetylation, Smith degradation, and 1H and 13C NMR spectroscopy, including 2D COSY, 1H-detected 1H, 13C heteronuclear single-quantum coherence (HSQC), and 1H-detected 1H, 13C heteronuclear multiple-bond connectivity (HMBC) experiments, the following struc...

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Bibliographic Details
Published in:Carbohydrate research 1997-03, Vol.298 (3), p.229-235
Main Authors: Arbatsky, N P, Shashkov, A S, Widmalm, G, Knirel, Y A, Zych, K, Sidorczyk, Z
Format: Article
Language:English
Subjects:
Acetylation
Alanine - chemistry
Carbohydrate Sequence
Gas Chromatography-Mass Spectrometry
Magnetic Resonance Spectroscopy
Methylation
Molecular Sequence Data
O Antigens - chemistry
Oligosaccharides - chemistry
Proteus - chemistry
Proteus - classification
Spectrometry, Mass, Fast Atom Bombardment
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Summary:Based on sugar and methylation analyses, O-deacetylation, Smith degradation, and 1H and 13C NMR spectroscopy, including 2D COSY, 1H-detected 1H, 13C heteronuclear single-quantum coherence (HSQC), and 1H-detected 1H, 13C heteronuclear multiple-bond connectivity (HMBC) experiments, the following structure of the O-specific polysaccharide of Proteus penneri strain 25 was established: [formula: see text] where D-GlcN(L-Ala) is 2-(L-alanylamido)-2-deoxy-D-glucose.
ISSN:0008-6215
DOI:10.1016/S0008-6215(96)00308-4